Innate Immune Evasion of Porcine Epidemic Diarrhea Virus through Degradation of the FBXW7 Protein via the Ubiquitin-Proteasome Pathway.
Animals
Antiviral Agents
/ immunology
Chlorocebus aethiops
Coronavirus Infections
/ immunology
F-Box-WD Repeat-Containing Protein 7
/ metabolism
Immune Evasion
Immunity, Innate
Interferon Type I
/ metabolism
Porcine epidemic diarrhea virus
/ immunology
Proteasome Endopeptidase Complex
/ metabolism
Signal Transduction
/ immunology
Swine
Swine Diseases
/ immunology
Ubiquitins
/ metabolism
Vero Cells
F-box and WD repeat domain-containing 7 protein
PEDV
coronavirus
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
09 03 2022
09 03 2022
Historique:
pubmed:
9
9
2021
medline:
15
3
2022
entrez:
8
9
2021
Statut:
ppublish
Résumé
Porcine epidemic diarrhea virus (PEDV) causes a porcine disease associated with swine epidemic diarrhea. Different antagonistic strategies have been identified, and the mechanism by which PEDV infection impairs the production of interferon (IFN) and delays the activation of the IFN response to escape host innate immunity has been determined, but the pathogenic mechanisms of PEDV infection remain enigmatic. Our preliminary results revealed that endogenous F-box and WD repeat domain-containing 7 (FBXW7) protein, the substrate recognition component of the SCF-type E3 ubiquitin ligase, is downregulated in PEDV-infected Vero E6 cells, according to the results from an isobaric tags for relative and absolute quantification (iTRAQ) analysis. Overexpression of FBXW7 in target cells makes them more resistant to PEDV infection, whereas ablation of FBXW7 expression by small interfering RNA (siRNA) significantly promotes PEDV infection. In addition, FBXW7 was verified as an innate antiviral factor capable of enhancing the expression of RIG-I and TBK1, and it was found to induce interferon-stimulated genes (ISGs), which led to an elevated antiviral state of the host cells. Moreover, we revealed that PEDV nonstructural protein 2 (nsp2) interacts with FBXW7 and targets FBXW7 for degradation through the K48-linked ubiquitin-proteasome pathway. Consistent with the results proven
Identifiants
pubmed: 34495699
doi: 10.1128/JVI.00889-21
pmc: PMC8906424
doi:
Substances chimiques
Antiviral Agents
0
F-Box-WD Repeat-Containing Protein 7
0
Interferon Type I
0
Ubiquitins
0
Proteasome Endopeptidase Complex
EC 3.4.25.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0088921Subventions
Organisme : National Key Research and Development Program of China
ID : 2021YFD1801105
Organisme : Natural Science Foundation of Heilongjiang Province of China
ID : YQ2020C023
Organisme : Central Public-interest Scientific Institution Basal Research Fund
ID : 1610302020001
Organisme : National Natural Science Foundation of China (NSFC)
ID : 31872474
Références
J Gen Virol. 2013 Jul;94(Pt 7):1554-1567
pubmed: 23596270
Vet Rec. 1977 Mar 19;100(12):243-4
pubmed: 888300
Front Immunol. 2018 May 28;9:1135
pubmed: 29892288
Cell Host Microbe. 2017 Aug 9;22(2):176-184
pubmed: 28799903
J Virol. 2017 Apr 28;91(10):
pubmed: 28250121
J Mol Biol. 2014 Mar 20;426(6):1246-64
pubmed: 24316048
Nat Rev Immunol. 2014 Jan;14(1):36-49
pubmed: 24362405
J Virol. 2016 Aug 26;90(18):8281-92
pubmed: 27384656
J Virol. 2014 Aug;88(16):8936-45
pubmed: 24872591
Emerg Infect Dis. 2012 Aug;18(8):1350-3
pubmed: 22840964
Annu Rev Biochem. 1998;67:227-64
pubmed: 9759489
J Biol Chem. 2001 Sep 21;276(38):35847-53
pubmed: 11461910
PLoS Pathog. 2019 Aug 21;15(8):e1007983
pubmed: 31433824
Front Immunol. 2017 Jan 03;7:662
pubmed: 28096803
Arch Virol. 2017 Aug;162(8):2415-2419
pubmed: 28470417
Biochem Biophys Res Commun. 2016 Feb 12;470(3):697-703
pubmed: 26774344
Curr Opin Virol. 2019 Feb;34:39-49
pubmed: 30654269
Infect Genet Evol. 2014 Jan;21:205-13
pubmed: 24291225
J Virol. 2014 Sep;88(18):10448-58
pubmed: 24965453
Mol Cell. 2003 Aug;12(2):381-92
pubmed: 14536078
J Immunol. 2015 Aug 15;195(4):1782-90
pubmed: 26179906
Emerg Infect Dis. 2012 Jan;18(1):161-3
pubmed: 22261231
Oncotarget. 2017 Jul 25;8(30):49655-49670
pubmed: 28591694
EMBO J. 2013 Oct 16;32(20):2751-63
pubmed: 24065129
Mol Cell. 2005 Sep 16;19(6):727-40
pubmed: 16153868
Nihon Juigaku Zasshi. 1983 Dec;45(6):829-32
pubmed: 6323804
Cell Host Microbe. 2013 Aug 14;14(2):125-35
pubmed: 23954152
Science. 2004 Feb 27;303(5662):1374-8
pubmed: 14739463
Nature. 2013 Jul 25;499(7459):388
pubmed: 23887408
Arch Virol. 2010 Sep;155(9):1471-6
pubmed: 20544234
Cell. 2010 Mar 19;140(6):805-20
pubmed: 20303872
Genes Dev. 2008 Jan 15;22(2):252-64
pubmed: 18198341
Virus Genes. 2012 Apr;44(2):167-75
pubmed: 22270324
J Virol. 2018 Mar 28;92(8):
pubmed: 29386292
Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):9085-90
pubmed: 15150404
FEBS Lett. 2017 Oct;591(20):3190-3210
pubmed: 28850669
Emerg Infect Dis. 2014 Jul;20(7):1223-6
pubmed: 24960370
J Virol. 2017 Apr 28;91(10):
pubmed: 28275187
Cell. 2011 Nov 11;147(4):728-41
pubmed: 22078875
PLoS Pathog. 2018 Jan 25;14(1):e1006838
pubmed: 29370303
Nat Commun. 2017 Mar 13;8:14654
pubmed: 28287082
Prog Nucleic Acid Res Mol Biol. 1986;33:19-56, 301
pubmed: 3025922
Arch Virol. 2016 Mar;161(3):537-47
pubmed: 26611909
Nature. 2008 Feb 28;451(7182):1069-75
pubmed: 18305538
Antiviral Res. 2017 Apr;140:76-82
pubmed: 28109912
Virology. 2017 Feb;502:33-38
pubmed: 27984784
J Virol. 2018 Oct 29;92(22):
pubmed: 30185587
Cancer Cell. 2014 Oct 13;26(4):455-64
pubmed: 25314076
Vet Microbiol. 2015 Jun 12;177(3-4):373-8
pubmed: 25843943
J Virol. 2019 Apr 3;93(8):
pubmed: 30728254
Mol Cancer. 2018 Aug 7;17(1):115
pubmed: 30086763
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1401-E1408
pubmed: 29378950
J Virol. 2015 Dec 09;90(4):2090-101
pubmed: 26656704
J Vet Med Sci. 2016 Mar;78(3):355-63
pubmed: 26537549
Nat Microbiol. 2019 Jun;4(6):914-924
pubmed: 30936491
J Gen Virol. 2008 Jul;89(Pt 7):1622-1632
pubmed: 18559932
Vet J. 2018 Jul;237:69-75
pubmed: 30089548
J Cell Biol. 2008 Jun 16;181(6):913-20
pubmed: 18559665
J Biol Chem. 2008 Nov 7;283(45):30540-8
pubmed: 18765672
Cell Res. 2019 Jan;29(1):67-79
pubmed: 30410068
Science. 2001 Oct 5;294(5540):173-7
pubmed: 11533444
J Virol. 2018 Jan 30;92(4):
pubmed: 29187542
Arch Virol. 2015 Aug;160(8):1931-8
pubmed: 26026958
Mol Immunol. 2019 Oct;114:100-107
pubmed: 31351410
J Biochem. 2012 Jan;151(1):5-11
pubmed: 21890623
J Virol. 2018 Jul 17;92(15):
pubmed: 29769346