CRISPR/Cas9-Based Split Fluorescent Protein Tagging.

Animals CRISPR-Associated Protein 9 / metabolism CRISPR-Cas Systems Gene Editing Green Fluorescent Proteins / genetics Zebrafish / genetics
CRISPR/Cas9 genetically encoded fluorescent protein tags knock-in split fluorescent protein

Journal

Zebrafish
ISSN: 1557-8542
Titre abrégé: Zebrafish
Pays: United States
ID NLM: 101225070

Informations de publication

Date de publication:
12 2021
Historique:
pubmed: 9 9 2021
medline: 15 3 2022
entrez: 8 9 2021
Statut: ppublish

Résumé

Genetically encoded fluorescent tags such as green fluorescent protein fused to protein have revolutionized cell biology as they permit high-resolution protein imaging in live systems. Split fluorescent proteins, with a small fragment of 16 amino acids, can be inserted in the coding sequence to label proteins. We demonstrate successful integration of two bright and fast maturing split fluorescent proteins, mNeon green and sfCherry2, in zebrafish, and show that they are suitable for live imaging, including time-lapse series, and that they have a high signal-to-noise ratio. Furthermore, we show that CRISPR/Cas9 can be used to generate fluorescently tagged proteins

Identifiants

DOI: 10.1089/zeb.2021.0031 PMID: 34495758
pubmed: 34495758
doi: 10.1089/zeb.2021.0031
doi:

Substances chimiques

Green Fluorescent Proteins 147336-22-9
CRISPR-Associated Protein 9 EC 3.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

369-373

Auteurs

Gokul Kesavan (G)

Center for Regenerative Therapies TU Dresden, Technische Universität Dresden, Dresden, Germany.
ORCID: 0000-0002-2828-1460

Anja Machate (A)

Center for Regenerative Therapies TU Dresden, Technische Universität Dresden, Dresden, Germany.
ORCID: 0000-0001-7400-8054

Michael Brand (M)

Center for Regenerative Therapies TU Dresden, Technische Universität Dresden, Dresden, Germany.
ORCID: 0000-0001-5711-6512

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › CRISPR/Cas9-Based Split Fluorescent Protein Tagging.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines associées aux CRISPR › Protéine-9 associée à CRISPR › CRISPR/Cas9-Based Split Fluorescent Protein Tagging.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › CRISPR/Cas9-Based Split Fluorescent Protein Tagging.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › CRISPR/Cas9-Based Split Fluorescent Protein Tagging.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Protéines à fluorescence verte › CRISPR/Cas9-Based Split Fluorescent Protein Tagging.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Cypriniformes › Cyprinidae › Danio zébré › CRISPR/Cas9-Based Split Fluorescent Protein Tagging.