Whole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout.

Aeromonas salmonicida Animals Disease Resistance / genetics Fish Diseases / genetics Furunculosis / genetics Genome-Wide Association Study Gram-Negative Bacterial Infections / genetics Oncorhynchus mykiss / genetics Quantitative Trait Loci

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
08 09 2021
Historique:
received: 15 04 2021
accepted: 24 08 2021
entrez: 9 9 2021
pubmed: 10 9 2021
medline: 12 11 2021
Statut: epublish

Résumé

Aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis, has extensive negative effects on wild and farmed salmonids worldwide. Vaccination induces some protection under certain conditions but disease outbreaks occur even in vaccinated fish. Therefore, alternative disease control approaches are required to ensure the sustainable expansion of rainbow trout aquaculture. Selective breeding can be applied to enhance host resistance to pathogens. The present work used genome-wide association study (GWAS) to identify quantitative trait loci (QTL) associated with A. salmonicida resistance in rainbow trout. A total 798 rainbow trout exposed to A. salmonicida by bath challenge revealed 614 susceptible and 138 resistant fish. Genotyping was conducted using the 57 K single nucleotide polymorphism (SNP) array and the GWAS was performed for survival and time to death phenotypes. We identified a QTL on chromosome 16 and located positional candidate genes in the proximity of the most significant SNPs. In addition, samples from exposed fish were examined for expression of 24 immune-relevant genes indicating a systematic immune response to the infection. The present work demonstrated that resistance to A. salmonicida is moderately heritable with oligogenic architecture. These result will be useful for the future breeding programs for improving the natural resistance of rainbow trout against furunculosis.

Identifiants

DOI: 10.1038/s41598-021-97437-7 PMID: 34497310 PMC: PMC8426485
pubmed: 34497310
doi: 10.1038/s41598-021-97437-7
pii: 10.1038/s41598-021-97437-7
pmc: PMC8426485
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

17857

Informations de copyright

© 2021. The Author(s).

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Auteurs

Moonika H Marana (MH)

Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C., Copenhagen, Denmark. mmarana@sund.ku.dk.

Asma M Karami (AM)

Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C., Copenhagen, Denmark.

Jørgen Ødegård (J)

, Aquagen, Trondheim, Norway.

Shaozhi Zuo (S)

Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C., Copenhagen, Denmark.

Rzgar M Jaafar (RM)

Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C., Copenhagen, Denmark.

Heidi Mathiessen (H)

Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C., Copenhagen, Denmark.

Louise von Gersdorff Jørgensen (L)

Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C., Copenhagen, Denmark.

Per W Kania (PW)

Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C., Copenhagen, Denmark.

Inger Dalsgaard (I)

Institute of Aquatic Resources, Technical University of Denmark, Kgs. Lyngby, Denmark.

Torben Nielsen (T)

Aquasearch Ova ApS, Jelling, Denmark.

Kurt Buchmann (K)

Laboratory of Aquatic Pathobiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C., Copenhagen, Denmark.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Bâtonnets à Gram négatif facultativement anaérobies › Aeromonas › Aeromonas salmonicida › Whole-genome association study searching for...
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