Cells of the human intestinal tract mapped across space and time.
Adult
Aging
Animals
Child
Crohn Disease
/ pathology
Datasets as Topic
Enteric Nervous System
/ anatomy & histology
Epithelial Cells
/ cytology
Female
Fetus
/ anatomy & histology
Health
Humans
Intestines
/ cytology
Lymph Nodes
/ cytology
Mice
Mice, Inbred C57BL
Organogenesis
Receptors, IgG
/ metabolism
Signal Transduction
Spatio-Temporal Analysis
Time Factors
Journal
Nature
ISSN: 1476-4687
Titre abrégé: Nature
Pays: England
ID NLM: 0410462
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
24
11
2020
accepted:
26
07
2021
entrez:
9
9
2021
pubmed:
10
9
2021
medline:
18
9
2021
Statut:
ppublish
Résumé
The cellular landscape of the human intestinal tract is dynamic throughout life, developing in utero and changing in response to functional requirements and environmental exposures. Here, to comprehensively map cell lineages, we use single-cell RNA sequencing and antigen receptor analysis of almost half a million cells from up to 5 anatomical regions in the developing and up to 11 distinct anatomical regions in the healthy paediatric and adult human gut. This reveals the existence of transcriptionally distinct BEST4 epithelial cells throughout the human intestinal tract. Furthermore, we implicate IgG sensing as a function of intestinal tuft cells. We describe neural cell populations in the developing enteric nervous system, and predict cell-type-specific expression of genes associated with Hirschsprung's disease. Finally, using a systems approach, we identify key cell players that drive the formation of secondary lymphoid tissue in early human development. We show that these programs are adopted in inflammatory bowel disease to recruit and retain immune cells at the site of inflammation. This catalogue of intestinal cells will provide new insights into cellular programs in development, homeostasis and disease.
Identifiants
pubmed: 34497389
doi: 10.1038/s41586-021-03852-1
pii: 10.1038/s41586-021-03852-1
pmc: PMC8426186
doi:
Substances chimiques
FCGR2A protein, human
0
Receptors, IgG
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
250-255Subventions
Organisme : Medical Research Council
ID : MC_PC_17230
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S035842/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : MR/R006237/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 203151/Z/16/Z
Pays : United Kingdom
Organisme : European Research Council
Pays : International
Organisme : Wellcome Trust
ID : 206194
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 213555/Z/18/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 220268/Z/20/Z
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P028160/1
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021. The Author(s).
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