Single-Cell Analysis of the Neonatal Immune System Across the Gestational Age Continuum.
Antigen-Presenting Cells
/ immunology
Biomarkers
Cell Communication
Disease Susceptibility
/ immunology
Embryonic Development
/ immunology
Gene Expression Regulation
Gestational Age
Humans
Immune System Phenomena
Immunomodulation
Infant, Newborn
Premature Birth
Signal Transduction
Single-Cell Analysis
/ methods
T-Lymphocyte Subsets
/ immunology
Neonatal immunology
neonatal NK cells
neonatal T cells
neonatal antigen presenting cells
neonatal cytotoxic cells
prematurity
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2021
2021
Historique:
received:
24
05
2021
accepted:
02
08
2021
entrez:
9
9
2021
pubmed:
10
9
2021
medline:
21
12
2021
Statut:
epublish
Résumé
Although most causes of death and morbidity in premature infants are related to immune maladaptation, the premature immune system remains poorly understood. We provide a comprehensive single-cell depiction of the neonatal immune system at birth across the spectrum of viable gestational age (GA), ranging from 25 weeks to term. A mass cytometry immunoassay interrogated all major immune cell subsets, including signaling activity and responsiveness to stimulation. An elastic net model described the relationship between GA and immunome (R=0.85, p=8.75e-14), and unsupervised clustering highlighted previously unrecognized GA-dependent immune dynamics, including decreasing basal MAP-kinase/NFκB signaling in antigen presenting cells; increasing responsiveness of cytotoxic lymphocytes to interferon-α; and decreasing frequency of regulatory and invariant T cells, including NKT-like cells and CD8
Identifiants
pubmed: 34497610
doi: 10.3389/fimmu.2021.714090
pmc: PMC8420969
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
714090Subventions
Organisme : Doris Duke Charitable Foundation
ID : 2018100
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM138353
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM137936
Pays : United States
Informations de copyright
Copyright © 2021 Peterson, Hedou, Ganio, Stelzer, Feyaerts, Harbert, Adusumelli, Ando, Tsai, Tsai, Han, Ringle, Houghteling, Reiss, Lewis, Winn, Angst, Aghaeepour, Stevenson and Gaudilliere.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Front Immunol. 2019 Aug 30;10:2035
pubmed: 31543877
Neurol Res Int. 2012;2012:561494
pubmed: 22530124
Obstet Gynecol. 2011 Mar;117(3):603-609
pubmed: 21446208
Science. 2008 Dec 5;322(5907):1562-5
pubmed: 19056990
J Exp Med. 2008 Aug 4;205(8):1903-16
pubmed: 18663128
Clin Exp Immunol. 1998 Aug;113(2):220-8
pubmed: 9717971
Front Immunol. 2018 Jun 28;9:1478
pubmed: 30013556
J Exp Med. 1998 Sep 7;188(5):867-76
pubmed: 9730888
Nat Rev Immunol. 2008 Jul;8(7):523-32
pubmed: 18566595
Gastroenterology. 2010 Jan;138(1):325-35.e1-2
pubmed: 19747917
Pediatr Res. 2005 May;57(5 Pt 1):649-55
pubmed: 15718362
J Immunol. 2005 Dec 15;175(12):7791-5
pubmed: 16339512
J Immunol. 2019 Oct 1;203(7):1793-1807
pubmed: 31492740
Am J Epidemiol. 2012 Jun 1;175(11):1191-9
pubmed: 22508393
Eur J Cell Biol. 2001 Aug;80(8):554-61
pubmed: 11561906
Pediatr Res. 1995 Jun;37(6):806-11
pubmed: 7544455
Semin Immunopathol. 2020 Aug;42(4):397-412
pubmed: 32020337
Neural Regen Res. 2017 Jun;12(6):890-896
pubmed: 28761416
J AAPOS. 1999 Feb;3(1):26-32
pubmed: 10071898
Nature. 2004 Jul 8;430(6996):257-63
pubmed: 15241424
Pediatrics. 2016 Jul;138(1):
pubmed: 27302979
Nat Rev Immunol. 2013 Feb;13(2):101-17
pubmed: 23334244
Nat Immunol. 2019 Mar;20(3):301-312
pubmed: 30664737
J Infect Dis. 2007 Jan 15;195(2):296-302
pubmed: 17191175
Trends Immunol. 2014 Jul;35(7):299-310
pubmed: 24880460
Obstet Gynecol. 1997 Nov;90(5):815-8
pubmed: 9351770
Nat Rev Immunol. 2013 Mar;13(3):159-75
pubmed: 23435331
Annu Rev Immunol. 2020 Apr 26;38:229-247
pubmed: 31928469
Clin Exp Immunol. 1982 Jun;48(3):649-54
pubmed: 6180853
Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):7821-6
pubmed: 12060727
Nat Med. 2020 Apr;26(4):599-607
pubmed: 32094926
Lancet. 2001 Nov 17;358(9294):1699-700
pubmed: 11728550
Nat Mach Intell. 2020 Oct;2(10):619-628
pubmed: 33294774
Nat Rev Gastroenterol Hepatol. 2016 Oct;13(10):590-600
pubmed: 27534694
J Affect Disord. 2008 May;108(1-2):147-57
pubmed: 18067974
Cell Mol Immunol. 2020 Jul;17(7):693-704
pubmed: 32467619
J Exp Med. 2018 Feb 5;215(2):459-479
pubmed: 29339446
J Immunol. 2010 Oct 15;185(8):4896-903
pubmed: 20861353
Early Hum Dev. 2009 Nov;85(11):719-25
pubmed: 19765918
PLoS Pathog. 2013;9(10):e1003681
pubmed: 24130485
Nature. 1953 Oct 3;172(4379):603-6
pubmed: 13099277
Nat Commun. 2017 Sep 29;8(1):748
pubmed: 28963474
Am J Physiol Lung Cell Mol Physiol. 2015 Sep 15;309(6):L593-604
pubmed: 26163511
Bioinformatics. 2019 Jan 1;35(1):95-103
pubmed: 30561547
Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):3006-11
pubmed: 20133607
EBioMedicine. 2019 Jun;44:71-85
pubmed: 31129099
Dis Markers. 2012;33(1):51-60
pubmed: 22710869
Infect Immun. 2011 Apr;79(4):1588-96
pubmed: 21300777
Nat Protoc. 2015 Feb;10(2):316-33
pubmed: 25612231
Gut. 2013 Jan;62(1):73-82
pubmed: 22267598
J Immunol. 1983 Jan;130(1):153-6
pubmed: 6847879
Hum Immunol. 2015 May;76(5):329-338
pubmed: 25797206
J Clin Invest. 2019 May 30;129(9):3562-3577
pubmed: 31145102
J Biol Chem. 2011 Nov 4;286(44):37964-37975
pubmed: 21930700
Cell. 2018 Aug 23;174(5):1277-1292.e14
pubmed: 30142345
J Clin Invest. 2016 Feb;126(2):495-508
pubmed: 26690704
Science. 1994 Jun 3;264(5164):1415-21
pubmed: 8197455
Hum Immunol. 2010 Feb;71(2):151-7
pubmed: 19961891
J Surg Res. 2011 Jul;169(1):e51-7
pubmed: 21529841
Cytometry A. 2013 May;83(5):483-94
pubmed: 23512433
Mol Cell Endocrinol. 2011 Mar 15;335(1):2-13
pubmed: 20398732
Nat Commun. 2020 Mar 9;11(1):1284
pubmed: 32152273
Immunology. 2010 Oct;131(2):289-99
pubmed: 20545784
Front Immunol. 2019 Jun 26;10:1448
pubmed: 31293595
Mediators Inflamm. 2018 Sep 19;2018:4209359
pubmed: 30327582
Cell Mol Gastroenterol Hepatol. 2018 Apr 06;6(2):229-238.e1
pubmed: 30105286
Immunol Lett. 2006 Jan 15;102(1):106-9
pubmed: 16199095
Cell Rep. 2014 Nov 6;9(3):1075-88
pubmed: 25437561
Sci Transl Med. 2018 Apr 25;10(438):
pubmed: 29695455
Br J Ophthalmol. 2015 Jan;99(1):125-32
pubmed: 25355806
Alzheimer Dis Assoc Disord. 2006 Jan-Mar;20(1):63-72
pubmed: 16493239
Invest Ophthalmol Vis Sci. 2019 Sep 3;60(12):3813-3820
pubmed: 31525777
Pediatrics. 2011 May;127(5):817-26
pubmed: 21518717
Pediatr Dev Pathol. 2006 Jan-Feb;9(1):3-13
pubmed: 16808630
Nat Immunol. 2019 Sep;20(9):1110-1128
pubmed: 31406380
Pediatr Res. 2012 May;71(5):590-7
pubmed: 22398700
BMJ. 2017 Mar 28;356:j1039
pubmed: 28351838
Am J Physiol Gastrointest Liver Physiol. 2014 Jul 15;307(2):G177-86
pubmed: 24852566
Eur J Immunol. 2005 Feb;35(2):383-90
pubmed: 15682453
J Neonatal Perinatal Med. 2016 Sep 16;9(3):233-42
pubmed: 27589549
Front Pediatr. 2020 Jan 28;8:5
pubmed: 32047730
Biol Neonate. 2004;86(2):116-23
pubmed: 15205540
Lancet. 2008 Apr 12;371(9620):1247-58
pubmed: 18406859
Clin Perinatol. 2010 Jun;37(2):307-37
pubmed: 20569810
Pediatrics. 2010 Oct;126(4):e865-73
pubmed: 20876174
Cell Rep. 2016 Nov 15;17(8):2151-2160
pubmed: 27851975
J Immunol. 2013 Apr 15;190(8):3949-58
pubmed: 23487427
Am J Obstet Gynecol. 2004 Oct;191(4):1375-81
pubmed: 15507968
Mol Immunol. 2021 Feb;130:154-158
pubmed: 33358567
Invest Ophthalmol Vis Sci. 2009 Feb;50(2):878-84
pubmed: 18806296
Pediatr Res. 2005 Jul;58(1):121-4
pubmed: 15879290