High-resolution within-sewer SARS-CoV-2 surveillance facilitates informed intervention.
Building-scale monitoring
COVID-19
Composite sampler
RT-qPCR
SARS-CoV-2
Wastewater surveillance
Wastewater-based epidemiology
Journal
Water research
ISSN: 1879-2448
Titre abrégé: Water Res
Pays: England
ID NLM: 0105072
Informations de publication
Date de publication:
01 Oct 2021
01 Oct 2021
Historique:
received:
16
06
2021
revised:
24
08
2021
accepted:
25
08
2021
pubmed:
10
9
2021
medline:
6
10
2021
entrez:
9
9
2021
Statut:
ppublish
Résumé
To assist in the COVID-19 public health guidance on a college campus, daily composite wastewater samples were withdrawn at 20 manhole locations across the University of Colorado Boulder campus. Low-cost autosamplers were fabricated in-house to enable an economical approach to this distributed study. These sample stations operated from August 25th until November 23rd during the fall 2020 semester, with 1512 samples collected. The concentration of SARS-CoV-2 in each sample was quantified through two comparative reverse transcription quantitative polymerase chain reactions (RT-qPCRs). These methods were distinct in the utilization of technical replicates and normalization to an endogenous control. (1) Higher temporal resolution compensates for supply chain or other constraints that prevent technical or biological replicates. (2) The data normalized by an endogenous control agreed with the raw concentration data, minimizing the utility of normalization. The raw wastewater concentration values reflected SARS-CoV-2 prevalence on campus as detected by clinical services. Overall, combining the low-cost composite sampler with a method that quantifies the SARS-CoV-2 signal within six hours enabled actionable and time-responsive data delivered to key stakeholders. With daily reporting of the findings, wastewater surveillance assisted in decision making during critical phases of the pandemic on campus, from detecting individual cases within populations ranging from 109 to 2048 individuals to monitoring the success of on-campus interventions.
Identifiants
pubmed: 34500183
pii: S0043-1354(21)00808-3
doi: 10.1016/j.watres.2021.117613
pmc: PMC8402945
pii:
doi:
Substances chimiques
Waste Water
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
117613Informations de copyright
Copyright © 2021. Published by Elsevier Ltd.
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