Independent transcriptomic and proteomic regulation by type I and II protein arginine methyltransferases.

cell biology molecular biology

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
24 Sep 2021
Historique:
received: 25 06 2020
revised: 21 06 2021
accepted: 09 08 2021
entrez: 10 9 2021
pubmed: 11 9 2021
medline: 11 9 2021
Statut: epublish

Résumé

Protein arginine methyltransferases (PRMTs) catalyze the post-translational monomethylation (Rme1), asymmetric (Rme2a), or symmetric (Rme2s) dimethylation of arginine. To determine the cellular consequences of type I (Rme2a) and II (Rme2s) PRMTs, we developed and integrated multiple approaches. First, we determined total cellular dimethylarginine levels, revealing that Rme2s was ∼3% of total Rme2 and that this percentage was dependent upon cell type and PRMT inhibition status. Second, we quantitatively characterized

Identifiants

pubmed: 34505004
doi: 10.1016/j.isci.2021.102971
pii: S2589-0042(21)00939-1
pmc: PMC8417332
doi:

Types de publication

Journal Article

Langues

eng

Pagination

102971

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM037537
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM108646
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM126421
Pays : United States

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

M.T.B. is a co-founder of EpiCypher.

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Auteurs

Maxim I Maron (MI)

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Stephanie M Lehman (SM)

Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.

Sitaram Gayatri (S)

Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, TX, USA.
Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center, Smithville, TX, USA.
Graduate Program in Genetics and Epigenetics, The University of Texas MD Anderson UT Health Graduate School of Biomedical Sciences, Houston, TX 77030, USA.

Joseph D DeAngelo (JD)

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Subray Hegde (S)

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Benjamin M Lorton (BM)

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Yan Sun (Y)

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Dina L Bai (DL)

Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.

Simone Sidoli (S)

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Varun Gupta (V)

Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Matthew R Marunde (MR)

EpiCypher, Inc., Research Triangle Park, NC 27709, USA.

James R Bone (JR)

EpiCypher, Inc., Research Triangle Park, NC 27709, USA.

Zu-Wen Sun (ZW)

EpiCypher, Inc., Research Triangle Park, NC 27709, USA.

Mark T Bedford (MT)

Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, TX, USA.
Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center, Smithville, TX, USA.
Graduate Program in Genetics and Epigenetics, The University of Texas MD Anderson UT Health Graduate School of Biomedical Sciences, Houston, TX 77030, USA.

Jeffrey Shabanowitz (J)

Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.

Hongshan Chen (H)

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Donald F Hunt (DF)

Departments of Chemistry and Pathology, University of Virginia, Charlottesville, VA 22904, USA.

David Shechter (D)

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Classifications MeSH