Placental expression of RNU44, RNU48 and miR-16-5p: stability and relations with fetoplacental growth.
Journal
European journal of clinical nutrition
ISSN: 1476-5640
Titre abrégé: Eur J Clin Nutr
Pays: England
ID NLM: 8804070
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
03
11
2020
accepted:
24
08
2021
revised:
04
08
2021
pubmed:
12
9
2021
medline:
14
5
2022
entrez:
11
9
2021
Statut:
ppublish
Résumé
The current study aimed to identify suitable reference miRNA for placental miRNA expression analysis in a set of well-characterized and fetal-sex balanced small- (SGA) and appropriate- (AGA) for gestational age full-term singleton pregnancies. In this retrospective study, placental samples (n = 106) from 35 SGA (19 male and 16 female) and 71 AGA (30 male and 41 female) full-term singleton pregnancies were utilized. Placental transcript abundance of three widely used reference miRNAs [miR-16-5p and Small nucleolar RNAs (snoRNAs) RNU44 and RNU48] were assessed by real-time quantitative PCR. Raw cycle threshold (C Raw C We recommend miR-16-5p and RNU44 as suitable reference miRNAs for placental samples from settings similar to our study.
Sections du résumé
BACKGROUND/OBJECTIVES
The current study aimed to identify suitable reference miRNA for placental miRNA expression analysis in a set of well-characterized and fetal-sex balanced small- (SGA) and appropriate- (AGA) for gestational age full-term singleton pregnancies.
SUBJECTS/METHODS
In this retrospective study, placental samples (n = 106) from 35 SGA (19 male and 16 female) and 71 AGA (30 male and 41 female) full-term singleton pregnancies were utilized. Placental transcript abundance of three widely used reference miRNAs [miR-16-5p and Small nucleolar RNAs (snoRNAs) RNU44 and RNU48] were assessed by real-time quantitative PCR. Raw cycle threshold (C
RESULTS
Raw C
CONCLUSION
We recommend miR-16-5p and RNU44 as suitable reference miRNAs for placental samples from settings similar to our study.
Identifiants
pubmed: 34508256
doi: 10.1038/s41430-021-01003-3
pii: 10.1038/s41430-021-01003-3
doi:
Substances chimiques
MIRN16 microRNA, human
0
MicroRNAs
0
RNA, Small Nucleolar
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
722-729Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature Limited.
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