Monitoring the cellular metabolism of a membrane-permeant photo-caged phosphatidylinositol 3,4,5-trisphosphate derivative.

Mass spectrometry Multi-reaction monitoring Phosphoinositides Photoactivatable groups Protecting groups

Journal

Chemistry and physics of lipids
ISSN: 1873-2941
Titre abrégé: Chem Phys Lipids
Pays: Ireland
ID NLM: 0067206

Informations de publication

Date de publication:
11 2021
Historique:
received: 02 06 2021
revised: 22 07 2021
accepted: 13 08 2021
pubmed: 13 9 2021
medline: 28 12 2021
entrez: 12 9 2021
Statut: ppublish

Résumé

To deliver charged lipid derivatives to the cell interior, bioactivatable and photo-activatable protecting groups are frequently used. The intracellular metabolism of the protecting groups, as well as the lipid itself, are key factors that determine biological activity. Here we followed the cellular metabolism of cell-permeant photo-activatable ("caged") and non-caged membrane-permeant analogs of dioctanoyl phosphatidylinositol 3,4,5-trisphosphate (diC

Identifiants

pubmed: 34509466
pii: S0009-3084(21)00077-3
doi: 10.1016/j.chemphyslip.2021.105124
pii:
doi:

Substances chimiques

Phosphatidylinositol Phosphates 0
phosphatidylinositol 3,4,5-triphosphate 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

105124

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM127631
Pays : United States

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Mevlut Citir (M)

European Molecular Biology Laboratory (EMBL), Cell Biology & Biophysics Unit, Meyerhofstraße 1, 69117, Heidelberg, Germany. Electronic address: mevcit@gmail.com.

Rainer Müller (R)

European Molecular Biology Laboratory (EMBL), Cell Biology & Biophysics Unit, Meyerhofstraße 1, 69117, Heidelberg, Germany. Electronic address: rmueller@embl.de.

Sebastian Hauke (S)

European Molecular Biology Laboratory (EMBL), Cell Biology & Biophysics Unit, Meyerhofstraße 1, 69117, Heidelberg, Germany. Electronic address: sebastian.hauke89@gmx.de.

Alexis Traynor-Kaplan (A)

ATK Innovation, Analytics and Discovery, North Bend, WA, 98045, USA; University of Washington, Department of Medicine, Seattle, WA, 98195, USA. Electronic address: atkaplan@u.washington.edu.

Carsten Schultz (C)

European Molecular Biology Laboratory (EMBL), Cell Biology & Biophysics Unit, Meyerhofstraße 1, 69117, Heidelberg, Germany; Oregon Health & Science University (OHSU), Department of Chemical Physiology and Biochemistry, L334 3181 SW Sam Jackson Park Road, Portland, OR, 97239-3098, USA. Electronic address: schulcar@ohsu.edu.

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Classifications MeSH