Generation of an Akaluc knock-in human embryonic stem cell reporter line using CRISPR-Cas9 technology.
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
07
07
2021
revised:
15
08
2021
accepted:
31
08
2021
pubmed:
13
9
2021
medline:
26
11
2021
entrez:
12
9
2021
Statut:
ppublish
Résumé
Akaluc, an enzyme engineered from luciferase, provides a potential powerful tool for tracing transplanted cells in vivo because of its near-infrared emission light. To enable evaluation of its potency, we inserted the Akaluc gene at AAVS1 locus using CRISPR/Cas9 technology and generated a clonal human embryonic stem stable cell line (Named H1-AAVS1-EF1α-Akaluc-KI or AkalucHES). AkalucHES could efficiently express Akaluc and were traced easily in vivo. We verified that AkalucHES expressed the pluripotency markers and showed normal stem cell morphology. Furthermore, AkalucHES maitains normal karyotype and is able to differentiate toward three germ-layer in vivo. So the Akaluc is effective for tracing transplanted cells in vivo.
Identifiants
pubmed: 34509917
pii: S1873-5061(21)00379-2
doi: 10.1016/j.scr.2021.102532
pii:
doi:
Substances chimiques
Luciferases
EC 1.13.12.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102532Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.