Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization.

5-aminolevulinic acid Epidermal stem cells Photodynamic therapy Re-epithelialization Transient amplifying cells Wound healing

Journal

Burns & trauma
ISSN: 2321-3868
Titre abrégé: Burns Trauma
Pays: England
ID NLM: 101651457

Informations de publication

Date de publication:
2021
Historique:
received: 17 12 2020
revised: 01 02 2021
entrez: 13 9 2021
pubmed: 14 9 2021
medline: 14 9 2021
Statut: epublish

Résumé

Epidermal stem cells (EpSCs) that reside in cutaneous hair follicles and the basal layer of the epidermis are indispensable for wound healing and skin homeostasis. Little is known about the effects of photochemical activation on EpSC differentiation, proliferation and migration during wound healing. The present study aimed to determine the effects of photodynamic therapy (PDT) on wound healing We created mouse full-thickness skin resection models and applied 5-aminolevulinic acid (ALA) for PDT to the wound beds. Wound healing was analysed by gross evaluation and haematoxylin-eosin staining PDT accelerated wound closure by enhancing EpSC differentiation, proliferation and migration, thereby promoting re-epithelialization and angiogenesis. PDT inhibited inflammatory infiltration and expression of proinflammatory cytokines, whereas the secretion of growth factors was greater than in other groups. The proportion of transient amplifying cells was significantly greater Topical ALA-induced PDT stimulates wound healing by enhancing re-epithelialization, promoting angiogenesis as well as modulating skin homeostasis. This work provides a preliminary theoretical foundation for the clinical administration of topical ALA-induced PDT in skin wound healing.

Sections du résumé

BACKGROUND BACKGROUND
Epidermal stem cells (EpSCs) that reside in cutaneous hair follicles and the basal layer of the epidermis are indispensable for wound healing and skin homeostasis. Little is known about the effects of photochemical activation on EpSC differentiation, proliferation and migration during wound healing. The present study aimed to determine the effects of photodynamic therapy (PDT) on wound healing
METHODS METHODS
We created mouse full-thickness skin resection models and applied 5-aminolevulinic acid (ALA) for PDT to the wound beds. Wound healing was analysed by gross evaluation and haematoxylin-eosin staining
RESULTS RESULTS
PDT accelerated wound closure by enhancing EpSC differentiation, proliferation and migration, thereby promoting re-epithelialization and angiogenesis. PDT inhibited inflammatory infiltration and expression of proinflammatory cytokines, whereas the secretion of growth factors was greater than in other groups. The proportion of transient amplifying cells was significantly greater
CONCLUSIONS CONCLUSIONS
Topical ALA-induced PDT stimulates wound healing by enhancing re-epithelialization, promoting angiogenesis as well as modulating skin homeostasis. This work provides a preliminary theoretical foundation for the clinical administration of topical ALA-induced PDT in skin wound healing.

Identifiants

DOI: 10.1093/burnst/tkab008 PMID: 34514005 PMC: PMC8420953
pubmed: 34514005
doi: 10.1093/burnst/tkab008
pii: tkab008
pmc: PMC8420953
doi:

Types de publication

Journal Article

Langues

eng

Pagination

tkab008

Informations de copyright

© The Author(s) 2021. Published by Oxford University Press.

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Auteurs

Zengjun Yang (Z)

Department of Dermatology, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.

Xiaohong Hu (X)

State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.

Lina Zhou (L)

Department of Endocrinology, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.

Yaxiong He (Y)

Department of Dermatology, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.

Xiaorong Zhang (X)

State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.

Jiacai Yang (J)

State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.

Zhenyu Ju (Z)

Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, No. 601 Huangpu Street, Tianhe District, Guangzhou, Guangdong Province, 510632, China.

Yih-Cherng Liou (YC)

Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543, Singapore.

Han-Ming Shen (HM)

Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 14 Science Drive 4, 117543, Singapore.

Gaoxing Luo (G)

State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.

Michael R Hamblin (MR)

Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, 40 Blossom Street, Boston, MA, 02114, USA.

Weifeng He (W)

State Key Laboratory of Trauma, Burn and Combined Injury, Institute of Burn Research, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.

Rui Yin (R)

Department of Dermatology, Southwest Hospital, Third Military Medical University (Army Medical University), No. 30 Gaotanyan Street, Shapingba District, Chongqing, 400038, China.

Classifications MeSH