Simultaneous Exposure to Intracellular and Extracellular Photosensitizers for the Treatment of Staphylococcus aureus Infections.
Staphylococcus aureus
antibodies
antimicrobial photodynamic therapy
iron-regulated surface determinant system
photosensitizer
small molecules
Journal
Antimicrobial agents and chemotherapy
ISSN: 1098-6596
Titre abrégé: Antimicrob Agents Chemother
Pays: United States
ID NLM: 0315061
Informations de publication
Date de publication:
17 11 2021
17 11 2021
Historique:
pubmed:
14
9
2021
medline:
15
12
2021
entrez:
13
9
2021
Statut:
ppublish
Résumé
Staphylococcus aureus is a serious threat to public health due to the rise of antibiotic resistance in this organism, which can prolong or exacerbate skin and soft tissue infections (SSTIs). Methicillin-resistant S. aureus is a Gram-positive bacterium and a leading cause of SSTIs. As such, many efforts are under way to develop therapies that target essential biological processes in S. aureus. Antimicrobial photodynamic therapy is an effective alternative to antibiotics; therefore we developed an approach to simultaneously expose S. aureus to intracellular and extracellular photosensitizers. A near infrared photosensitizer was conjugated to human monoclonal antibodies (MAbs) that target the S. aureus iron-regulated surface determinant (Isd) heme acquisition proteins. In addition, the compound VU0038882 was developed to increase photoactivatable porphyrins within the cell. Combinatorial photodynamic treatment of drug-resistant S. aureus exposed to VU0038882 and conjugated anti-Isd MAbs proved to be an effective antibacterial strategy
Identifiants
pubmed: 34516248
doi: 10.1128/AAC.00919-21
pmc: PMC8597735
doi:
Substances chimiques
Anti-Bacterial Agents
0
Photosensitizing Agents
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0091921Subventions
Organisme : NIAID NIH HHS
ID : R01 AI069233
Pays : United States
Organisme : U.S. Department of Defense (DOD)
ID : W81XWH-17-2-0003
Organisme : HHS | National Institutes of Health (NIH)
ID : T32GM065086
Organisme : HHS | National Institutes of Health (NIH)
ID : R0169233
Organisme : NIGMS NIH HHS
ID : T32 GM065086
Pays : United States
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