Passive sampling, a practical method for wastewater-based surveillance of SARS-CoV-2.
COVID-19
Passive samplers
RT-qPCR
SARS-CoV-2
Wastewater surveillance
wastewater-based epidemiology (WBE)
Journal
Environmental research
ISSN: 1096-0953
Titre abrégé: Environ Res
Pays: Netherlands
ID NLM: 0147621
Informations de publication
Date de publication:
03 2022
03 2022
Historique:
received:
21
07
2021
revised:
09
09
2021
accepted:
10
09
2021
pubmed:
14
9
2021
medline:
18
12
2021
entrez:
13
9
2021
Statut:
ppublish
Résumé
In search of practical and affordable tools for wastewater-based surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), three independent field experiments were conducted using three passive sampler sorbents (electronegative membrane, cotton bud, and gauze) in Guelph, Ontario, Canada. Total daily cases during this study ranged from 2 to 17/100,000 people and 43/54 traditionally collected wastewater samples were positive for SARS-CoV-2 with mean detectable concentrations ranging from 8.4 to 1780 copies/ml. Viral levels on the passive samplers were assessed after 4, 8, 24, 48, 72, and 96 hrs of deployment in the wastewater and 43/54 membrane, 42/54 gauze, and 27/54 cotton bud samples were positive. A linear accumulation rate of SARS-CoV-2 on the membranes was observed up to 48 hours, suggesting the passive sampler could adequately reflect wastewater levels for up to two days of deployment. Due the variability in accumulation observed for the cotton buds and gauzes, and the pre-processing steps required for the gauzes, we recommend membrane filters as a simple cost-effective option for wastewater-based surveillance of SARS-CoV-2.
Identifiants
pubmed: 34516976
pii: S0013-9351(21)01353-0
doi: 10.1016/j.envres.2021.112058
pmc: PMC8433097
pii:
doi:
Substances chimiques
Waste Water
0
Types de publication
Letter
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112058Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Références
mSystems. 2020 Jul 21;5(4):
pubmed: 32694130
JAMA Intern Med. 2020 Nov 01;180(11):1447-1452
pubmed: 32780793
Appl Environ Microbiol. 2009 Nov;75(22):7261-7
pubmed: 19767474
Virus Res. 2020 Nov;289:198147
pubmed: 32866537
Sci Total Environ. 2020 Oct 15;739:139076
pubmed: 32758929
Lancet Microbe. 2021 Jan;2(1):e13-e22
pubmed: 33521734
Environ Res. 2021 Apr;195:110831
pubmed: 33587948
Sci Total Environ. 2021 Jun 10;772:145268
pubmed: 33556806
Front Med. 2020 Apr;14(2):185-192
pubmed: 32170560
Sci Total Environ. 2022 Feb 10;807(Pt 3):151047
pubmed: 34673061
Water Res. 2021 Sep 1;202:117400
pubmed: 34274898
BMJ. 2020 Apr 21;369:m1443
pubmed: 32317267
Sci Total Environ. 2021 Oct 10;790:148205
pubmed: 34102442
Environ Sci Technol. 2021 Aug 3;55(15):10432-10441
pubmed: 34264643
Plant Methods. 2014 Dec 31;10(1):42
pubmed: 25628753
JAMA Netw Open. 2020 Jun 1;3(6):e2011335
pubmed: 32525549
Environ Sci Technol. 2017 Aug 1;51(15):8692-8700
pubmed: 28657725
Water Res. 2021 Jun 15;198:117183
pubmed: 33962244
Nat Biotechnol. 2020 Oct;38(10):1164-1167
pubmed: 32948856
Sci Rep. 2020 Dec 29;10(1):22419
pubmed: 33376251