Unsaturated lipid isomeric imaging based on the Paternò-Büchi reaction in the solid phase in ambient conditions.

Animals Diagnostic Imaging Isomerism Lipids Mice Spectrometry, Mass, Electrospray Ionization
Air-flow-assisted desorption electrospray ionisation Lipids Mass spectrometry imaging Paternò–BüChi reaction Solid phase Spraying

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
01 Dec 2021
Historique:
received: 02 06 2021
revised: 17 08 2021
accepted: 18 08 2021
entrez: 14 9 2021
pubmed: 15 9 2021
medline: 16 9 2021
Statut: ppublish

Résumé

In recent years, the development of unsaturated lipid isomeric imaging based on the Paternò-Büchi (PB) reaction has improved significantly. However, research on this imaging method in ambient conditions needs to expand. In this paper, a method of PB reaction in the solid phase in ambient conditions is developed, which is combined with air-flow-assisted desorption electrospray ionisation mass spectrometry (AFADESI-MS) to achieve in situ imaging of lipids at an isomeric level. Experiments showed that the efficiency of the PB reaction was much higher when spraying the reagent solution than when sprinkling the reactant powder directly, and it was not lower than that in the liquid phase. This method can simplify the reaction conditions in the imaging process and can be applied to tissue section samples with only 10 min of pre-processing. The study successfully demonstrated the spatial distribution of unsaturated lipid isomers, and the isomeric ratio corresponded to the lesion areas in mouse brain cancer tissues. Due to its simple operation and performance in ambient conditions, this method may be useful for future studies on lipid isomers in tissues.

Identifiants

DOI: 10.1016/j.talanta.2021.122816 PMID: 34517672
pubmed: 34517672
pii: S0039-9140(21)00737-2
doi: 10.1016/j.talanta.2021.122816
pii:
doi:

Substances chimiques

Lipids 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

122816

Informations de copyright

Copyright © 2021 Elsevier B.V. All rights reserved.

Auteurs

Jian Zhang (J)

School of Life Science, Beijing Institute of Technology, No.5 Yard, Zhongguancun South Street, Haidian District, Beijing, 100081, China; State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Zhongguancun Street, Haidian District, Beijing, 100084, China.

Chengan Guo (C)

State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Zhongguancun Street, Haidian District, Beijing, 100084, China.

Xinming Huo (X)

Tsinghua Shenzhen International Graduate School, Shenzhen University Town, Lishui Road, Xili Town, Nanshan District, Shenzhen, 518055, China.

Xiaoxiao Ma (X)

State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Zhongguancun Street, Haidian District, Beijing, 100084, China.

Xin Li (X)

State Key Laboratory of Bioactive Substances and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, A2 Nanwei Road, Xicheng District, Beijing, 100050, China.

Zeper Abliz (Z)

State Key Laboratory of Bioactive Substances and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, A2 Nanwei Road, Xicheng District, Beijing, 100050, China; Centre for Imaging and Systems Biology, Minzu University of China, No.27 Yard, Zhongguancun South Street, Haidian District, Beijing, 100091, China.

Yao Chu (Y)

State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Zhongguancun Street, Haidian District, Beijing, 100084, China.

Xiaohao Wang (X)

State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Zhongguancun Street, Haidian District, Beijing, 100084, China.

Fei Tang (F)

State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instruments, Tsinghua University, Zhongguancun Street, Haidian District, Beijing, 100084, China. Electronic address: tangf@mail.tsinghua.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Unsaturated lipid isomeric imaging based on...
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Imagerie diagnostique › Unsaturated lipid isomeric imaging based on...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes de chimie organique › Isomérie › Unsaturated lipid isomeric imaging based on...
questionsmedicales.fr › Lipides › Unsaturated lipid isomeric imaging based on...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Unsaturated lipid isomeric imaging based on...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse ESI › Unsaturated lipid isomeric imaging based on...