Exosome-mediated lncRNA SNHG11 regulates angiogenesis in pancreatic carcinoma through miR-324-3p/VEGFA axis.
Animals
Apoptosis
Cell Line, Tumor
Cell Movement
Cell Proliferation
Exosomes
/ genetics
Gene Expression Regulation, Neoplastic
Humans
Mice, Inbred BALB C
Mice, Nude
MicroRNAs
/ genetics
Neoplasm Invasiveness
Neovascularization, Pathologic
Pancreatic Neoplasms
/ genetics
RNA, Long Noncoding
/ genetics
Signal Transduction
Vascular Endothelial Growth Factor A
/ genetics
Pancreatic Neoplasms
VEGFA
angiogenesis
exosome
lncRNA SNHG11
miR-324-3p
pancreatic carcinoma
Journal
Cell biology international
ISSN: 1095-8355
Titre abrégé: Cell Biol Int
Pays: England
ID NLM: 9307129
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
revised:
27
08
2021
received:
25
04
2021
accepted:
12
09
2021
pubmed:
15
9
2021
medline:
19
3
2022
entrez:
14
9
2021
Statut:
ppublish
Résumé
Pancreatic carcinoma (PC) is one of the most common and deadly human malignancies worldwide. LncRNAs play significant roles in the occurrence and development of various cancers. LncRNA SNHG11 (SNHG11) has been found to display high expression in serum of PC patients, which implies that dysregulated SNHG11 may be related to the development of PC. However, there is still a knowledge gap concerning the specific function and molecular mechanism of SNHG11 in PC. After conducting experiments with constructed models in vitro or in vivo, we found that exosomal SNHG11 promoted cell proliferation, migration, and angiogenesis but impeded cell apoptosis in PC in vitro, and additionally, it facilitated tumor growth in vivo. Exosome-mediated SNHG11 regulated the expression of VEGFA through sponging miR-324-3p. Rescue assays validated that the inhibitory effect of SNHG11 depletion on cell proliferation, migration, and angiogenesis could be reversed by miR-324-3p downregulation or VEGFA upregulation, and the promoting effect of SNHG11 silence on cell apoptosis could be rescued by transfection of miR-324-3p inhibitor or pcDNA3.1-VEGFA. To conclude, exosomal-mediated SNHG11 could regulate PC progression via miR-324-3p/VEGFA axis. Our findings may provide a novel insight for understanding PC, which might contribute to the development of potential PC biomarker.
Substances chimiques
MIRN324 microRNA, human
0
MicroRNAs
0
RNA, Long Noncoding
0
VEGFA protein, human
0
Vascular Endothelial Growth Factor A
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106-117Subventions
Organisme : CD146 acts as a receptor binding netrin-1 to activate NF-κB signaling pathway to induce EMT and promote the progression of colorectal cancer
ID : 2020YJKT05
Informations de copyright
© 2021 International Federation for Cell Biology.
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