Low-Cost, Open-Source Device for High-Performance Fluorescence Detection of Isothermal Nucleic Acid Amplification Reactions.

COVID-19 / diagnosis Humans Molecular Diagnostic Techniques Nucleic Acid Amplification Techniques RNA, Viral / genetics SARS-CoV-2
SARS-CoV-2 detection decentralized diagnostics loop-mediated isothermal amplification low-cost molecular diagnostics point of care diagnostics rapid diagnostic test

Journal

ACS biomaterials science & engineering
ISSN: 2373-9878
Titre abrégé: ACS Biomater Sci Eng
Pays: United States
ID NLM: 101654670

Informations de publication

Date de publication:
11 10 2021
Historique:
pubmed: 16 9 2021
medline: 15 10 2021
entrez: 15 9 2021
Statut: ppublish

Résumé

The ability to detect SARS-CoV-2 is critical to implementing evidence-based strategies to address the COVID-19 global pandemic. Expanding SARS-CoV-2 diagnostic ability beyond well-equipped laboratories widens the opportunity for surveillance and control efforts. However, such advances are predicated on the availability of rapid, scalable, accessible, yet high-performance diagnostic platforms. Methods to detect viral RNA using reverse transcription loop-mediated isothermal amplification (RT-LAMP) show promise as rapid and field-deployable tests; however, the per-unit costs of the required diagnostic hardware can be a barrier for scaled deployment. Here, we describe a diagnostic hardware configuration for LAMP technology, named the

Identifiants

DOI: 10.1021/acsbiomaterials.1c01105 PMID: 34521204
pubmed: 34521204
doi: 10.1021/acsbiomaterials.1c01105
doi:

Substances chimiques

RNA, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4982-4990

Auteurs

Andrew H Buultjens (AH)

Department of Microbiology and Immunology, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia.
ORCID: 0000-0002-5984-1328

Koen Vandelannoote (K)

Department of Microbiology and Immunology, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia.

Liam K Sharkey (LK)

Department of Microbiology and Immunology, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia.

Benjamin P Howden (BP)

Microbiological Diagnostic Unit Public Health Laboratory, Level 1, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia.
Doherty Applied Microbial Genomics, Department of Microbiology and Immunology, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia.
Department of Infectious Diseases, Austin Hospital, 145 Studley Road, Heidelberg 3084, Victoria, Australia.

Ian R Monk (IR)

Department of Microbiology and Immunology, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia.

Jean Y H Lee (JYH)

Department of Microbiology and Immunology, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia.
Department of Infectious Diseases, Monash Health, 246 Clayton Road, Clayton 3168, Victoria, Australia.

Timothy P Stinear (TP)

Department of Microbiology and Immunology, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia.
Doherty Applied Microbial Genomics, Department of Microbiology and Immunology, The University of Melbourne at The Doherty Institute for Infection and Immunity, 792 Elizabeth Street, Melbourne 3000, Victoria, Australia.
ORCID: 0000-0003-0150-123X

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Classifications MeSH

questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › Low-Cost, Open-Source Device for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Low-Cost, Open-Source Device for...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de diagnostic moléculaire › Low-Cost, Open-Source Device for...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Low-Cost, Open-Source Device for...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN viral › Low-Cost, Open-Source Device for...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › Virus du SRAS › SARS-CoV-2 › Low-Cost, Open-Source Device for...