Spatiotemporal beam self-cleaning for high-resolution nonlinear fluorescence imaging with multimode fiber.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 09 2021
14 09 2021
Historique:
received:
20
05
2021
accepted:
29
07
2021
entrez:
15
9
2021
pubmed:
16
9
2021
medline:
16
9
2021
Statut:
epublish
Résumé
Beam self-cleaning (BSC) in graded-index (GRIN) multimode fibers (MMFs) has been recently reported by different research groups. Driven by the interplay between Kerr effect and beam self-imaging, BSC counteracts random mode coupling, and forces laser beams to recover a quasi-single mode profile at the output of GRIN fibers. Here we show that the associated self-induced spatiotemporal reshaping allows for improving the performances of nonlinear fluorescence (NF) microscopy and endoscopy using multimode optical fibers. We experimentally demonstrate that the beam brightness increase, induced by self-cleaning, enables two and three-photon imaging of biological samples with high spatial resolution. Temporal pulse shortening accompanying spatial beam clean-up enhances the output peak power, hence the efficiency of nonlinear imaging. We also show that spatiotemporal supercontinuum (SC) generation is well-suited for large-band NF imaging in visible and infrared domains. We substantiated our findings by multiphoton fluorescence imaging in both microscopy and endoscopy configurations.
Identifiants
pubmed: 34521869
doi: 10.1038/s41598-021-96753-2
pii: 10.1038/s41598-021-96753-2
pmc: PMC8440782
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18240Informations de copyright
© 2021. The Author(s).
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