The nose is the best niche for detection of experimental pneumococcal colonisation in adults of all ages, using nasal wash.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 09 2021
14 09 2021
Historique:
received:
10
05
2021
accepted:
10
08
2021
entrez:
15
9
2021
pubmed:
16
9
2021
medline:
17
12
2021
Statut:
epublish
Résumé
Previous studies have suggested that the pneumococcal niche changes from the nasopharynx to the oral cavity with age. We use an Experimental Human Pneumococcal Challenge model to investigate pneumococcal colonisation in different anatomical niches with age. Healthy adults (n = 112) were intranasally inoculated with Streptococcus pneumoniae serotype 6B (Spn6B) and were categorised as young 18-55 years (n = 57) or older > 55 years (n = 55). Colonisation status (frequency and density) was determined by multiplex qPCR targeting the lytA and cpsA-6A/B genes in both raw and culture-enriched nasal wash and oropharyngeal swab samples collected at 2-, 7- and 14-days post-exposure. For older adults, raw and culture-enriched saliva samples were also assessed. 64% of NW samples and 54% of OPS samples were positive for Spn6B in young adults, compared to 35% of NW samples, 24% of OPS samples and 6% of saliva samples in older adults. Many colonisation events were only detected in culture-enriched samples. Experimental colonisation was detected in 72% of young adults by NW and 63% by OPS. In older adults, this was 51% by NW, 36% by OPS and 9% by saliva. The nose, as assessed by nasal wash, is the best niche for detection of experimental pneumococcal colonisation in both young and older adults.
Identifiants
pubmed: 34521967
doi: 10.1038/s41598-021-97807-1
pii: 10.1038/s41598-021-97807-1
pmc: PMC8440778
doi:
Substances chimiques
DNA, Bacterial
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18279Subventions
Organisme : Medical Research Council
ID : MR/M011569/1
Pays : United Kingdom
Informations de copyright
© 2021. The Author(s).
Références
J Infect Dis. 2012 Jan 15;205(2):280-8
pubmed: 22128315
BMC Res Notes. 2011 Apr 13;4:122
pubmed: 21489228
Vaccine. 2013 Dec 17;32(1):165-79
pubmed: 24331112
J Clin Microbiol. 2006 Feb;44(2):525-8
pubmed: 16455908
J Infect Dis. 2005 Aug 1;192(3):387-93
pubmed: 15995951
Vaccine. 2020 Feb 28;38(10):2298-2306
pubmed: 32035708
J Clin Microbiol. 2010 Aug;48(8):2762-9
pubmed: 20519466
Curr Opin Microbiol. 2018 Feb;41:8-14
pubmed: 29156371
AIDS. 2015 Sep 10;29(14):1837-44
pubmed: 26218599
J Clin Microbiol. 2007 Aug;45(8):2460-6
pubmed: 17537936
PLoS One. 2019 Aug 22;14(8):e0221525
pubmed: 31437226
Vaccine. 2019 Jul 9;37(30):3953-3956
pubmed: 31176540
J Am Geriatr Soc. 2015 Oct;63(10):2094-8
pubmed: 26456473
Am J Respir Crit Care Med. 2015 Oct 1;192(7):853-8
pubmed: 26114410
Lancet Infect Dis. 2004 Mar;4(3):144-54
pubmed: 14998500
PLoS One. 2020 Mar 10;15(3):e0229558
pubmed: 32155176
PLoS One. 2013;8(3):e60520
pubmed: 23555985
Science. 2012 Jun 8;336(6086):1255-62
pubmed: 22674335
J Am Geriatr Soc. 2010 Feb;58(2):396-8
pubmed: 20370873
Vaccine. 2016 Jan 2;34(1):4-6
pubmed: 26602269
Lancet Respir Med. 2014 Feb;2(2):141-53
pubmed: 24503269
Am J Med. 2018 Jan;131(1):100.e1-100.e7
pubmed: 28803139
Clin Infect Dis. 2012 Sep;55(6):816-24
pubmed: 22700830
Int J Infect Dis. 2019 Sep;86:73-81
pubmed: 31247341
PLoS One. 2015 Mar 19;10(3):e0119875
pubmed: 25789854
Am J Epidemiol. 2019 Dec 31;188(12):2110-2119
pubmed: 31509184
mBio. 2021 Jan 12;12(1):
pubmed: 33436429
Pediatr Infect Dis J. 2003 Feb;22(2):133-40
pubmed: 12586977
Am J Respir Crit Care Med. 2021 Mar 1;203(5):604-613
pubmed: 32941735
J Vis Exp. 2013 Feb 15;(72):
pubmed: 23439027
Expert Rev Vaccines. 2012 Jul;11(7):841-55
pubmed: 22913260
Sci Rep. 2019 Mar 1;9(1):3285
pubmed: 30824850
Expert Rev Vaccines. 2020 Apr;19(4):353-366
pubmed: 32237926
Open Forum Infect Dis. 2020 Aug 18;7(9):ofaa368
pubmed: 32995349
Am J Respir Crit Care Med. 2013 Apr 15;187(8):855-64
pubmed: 23370916
J Clin Microbiol. 2012 Sep;50(9):3146-7
pubmed: 22760044
Epidemiol Infect. 2004 Jun;132(3):433-41
pubmed: 15188713
J Clin Microbiol. 2004 Nov;42(11):4974-6
pubmed: 15528682
BMC Infect Dis. 2010 Oct 23;10:304
pubmed: 20969800
Pediatr Infect Dis J. 2016 Aug;35(8):907-14
pubmed: 27171679
Sci Rep. 2016 Oct 07;6:34888
pubmed: 27713565
PLoS One. 2010 Feb 19;5(2):e9282
pubmed: 20174571
J Infect Dis. 2019 May 24;219(12):1989-1993
pubmed: 30690468
J Infect. 2020 Oct;81(4):540-548
pubmed: 32562794
Clin Microbiol Infect. 2014 Dec;20(12):O1145-51
pubmed: 24995531
Sci Rep. 2015 Jun 12;5:11344
pubmed: 26067932
PeerJ. 2013 Jun 25;1:e97
pubmed: 23825797