The effect of absent blood flow on the zebrafish cerebral and trunk vasculature.
in vivo
light sheet
quantification
vasculature
zebrafish
Journal
Vascular biology (Bristol, England)
ISSN: 2516-5658
Titre abrégé: Vasc Biol
Pays: England
ID NLM: 101763329
Informations de publication
Date de publication:
2021
2021
Historique:
received:
20
05
2021
accepted:
29
07
2021
entrez:
15
9
2021
pubmed:
16
9
2021
medline:
16
9
2021
Statut:
epublish
Résumé
The role of blood flow in vascular development is complex and context-dependent. In this study, we quantify the effect of the lack of blood flow on embryonic vascular development on two vascular beds, namely the cerebral and trunk vasculature in zebrafish. We perform this by analysing vascular topology, endothelial cell (EC) number, EC distribution, apoptosis, and inflammatory response in animals with normal blood flow or absent blood flow. We find that absent blood flow reduced vascular area and EC number significantly in both examined vascular beds, but the effect is more severe in the cerebral vasculature, and severity increases over time. Absent blood flow leads to an increase in non-EC-specific apoptosis without increasing tissue inflammation, as quantified by cerebral immune cell numbers and nitric oxide. Similarly, while stereotypic vascular patterning in the trunk is maintained, intra-cerebral vessels show altered patterning, which is likely to be due to vessels failing to initiate effective fusion and anastomosis rather than sprouting or path-seeking. In conclusion, blood flow is essential for cellular survival in both the trunk and cerebral vasculature, but particularly intra-cerebral vessels are affected by the lack of blood flow, suggesting that responses to blood flow differ between these two vascular beds.
Identifiants
pubmed: 34522840
doi: 10.1530/VB-21-0009
pii: VB-21-0009
pmc: PMC8428019
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1-16Subventions
Organisme : British Heart Foundation
ID : PG/20/10017
Pays : United Kingdom
Informations de copyright
© 2021 The authors.
Références
Nature. 2018 Feb 22;554(7693):475-480
pubmed: 29443965
Genes Dev. 2009 Oct 1;23(19):2272-7
pubmed: 19797767
Physiol Genomics. 2009 Aug 7;38(3):319-27
pubmed: 19509081
J Imaging. 2019 Jan 11;5(1):
pubmed: 34465714
Dev Dyn. 1995 Jul;203(3):253-310
pubmed: 8589427
Cell Rep. 2015 Jun 23;11(11):1786-96
pubmed: 26074079
Cardiovasc Res. 2011 Jul 15;91(2):279-88
pubmed: 21602174
Neurobiol Dis. 2010 Jan;37(1):13-25
pubmed: 19664713
Cardiovasc Res. 2013 Nov 1;100(2):252-61
pubmed: 23812297
PLoS One. 2013 Oct 11;8(10):e75060
pubmed: 24146748
Arterioscler Thromb Vasc Biol. 2017 Jan;37(1):130-143
pubmed: 27834691
EMBO Rep. 2019 Aug;20(8):e47047
pubmed: 31379129
Cell Rep. 2014 Mar 13;6(5):799-808
pubmed: 24561257
Trends Cardiovasc Med. 2008 May;18(4):150-5
pubmed: 18555188
Blood. 2006 Dec 15;108(13):3976-8
pubmed: 16926288
Nature. 2003 Jan 9;421(6919):172-7
pubmed: 12520305
Acta Histochem. 2010 May;112(3):203-14
pubmed: 19481785
N Engl J Med. 1999 Sep 2;341(10):738-46
pubmed: 10471461
Cardiovasc Res. 2019 Aug 1;115(10):1449-1451
pubmed: 30924878
PLoS One. 2015 Jun 05;10(6):e0128878
pubmed: 26047145
Dev Biol. 2002 Aug 15;248(2):307-18
pubmed: 12167406
Front Cell Neurosci. 2015 Aug 31;9:321
pubmed: 26379496
Dev Biol. 2006 Feb 15;290(2):265-76
pubmed: 16405941
Circ Res. 2019 Oct 25;125(10):e43-e54
pubmed: 31495257
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Nat Cell Biol. 2016 Apr;18(4):443-50
pubmed: 26928868
Front Physiol. 2020 Jun 05;11:552
pubmed: 32581842
Elife. 2016 Feb 04;5:e07727
pubmed: 26845523
PLoS Biol. 2012;10(8):e1001374
pubmed: 22904685
PLoS Biol. 2015 Apr 17;13(4):e1002126
pubmed: 25884426
Genes Dev. 2008 Mar 15;22(6):734-9
pubmed: 18347092
Nat Commun. 2016 Jun 01;7:11805
pubmed: 27248505
Can J Cardiol. 2015 May;31(5):569-71
pubmed: 25795106
Semin Cell Dev Biol. 2011 Dec;22(9):1012-8
pubmed: 22008724
Circ Res. 2017 Feb 3;120(3):439-448
pubmed: 28154096
Thromb Haemost. 2011 May;105(5):811-9
pubmed: 21225092
Nat Genet. 2002 May;31(1):106-10
pubmed: 11967535
Dev Biol. 2010 Dec 1;348(1):34-46
pubmed: 20832394
Nat Rev Cardiol. 2020 Jan;17(1):52-63
pubmed: 31366922
PLoS Biol. 2015 May 15;13(5):e1002148
pubmed: 25978860
Dev Biol. 2015 Oct 1;406(1):40-51
pubmed: 26234750
Cardiovasc Res. 2013 Jul 15;99(2):276-83
pubmed: 23580605
Nat Commun. 2018 Dec 14;9(1):5314
pubmed: 30552331
Sci Rep. 2018 Jun 29;8(1):9840
pubmed: 29959335
PLoS Genet. 2017 Oct 19;13(10):e1007000
pubmed: 29049395
Development. 2011 May;138(9):1717-26
pubmed: 21429983
Dev Biol. 2008 Apr 15;316(2):312-22
pubmed: 18342303
Trends Genet. 2002 Oct;18(10):491-4
pubmed: 12350332
Methods Cell Biol. 2011;101:19-38
pubmed: 21550438
Front Neurosci. 2014 Nov 06;8:355
pubmed: 25414634
N Engl J Med. 2011 Feb 17;364(7):656-65
pubmed: 21323543
Elife. 2018 Feb 05;7:
pubmed: 29400648
Development. 2019 Aug 27;146(16):
pubmed: 31375478
Arterioscler Thromb Vasc Biol. 2017 Nov;37(11):2087-2101
pubmed: 28882872
Dev Biol. 2001 Feb 15;230(2):278-301
pubmed: 11161578
Science. 2004 Aug 13;305(5686):1007-9
pubmed: 15310904
Development. 2003 Nov;130(21):5281-90
pubmed: 12954720
Elife. 2015 Jun 08;4:
pubmed: 26051822
Anal Chem. 1998 Jul 1;70(13):2446-53
pubmed: 9666719
Trends Cardiovasc Med. 1994 Sep-Oct;4(5):207-12
pubmed: 21244869
Dev Cell. 2016 Jul 25;38(2):214-22
pubmed: 27424497
Prog Mol Biol Transl Sci. 2014;124:155-88
pubmed: 24751430
J Clin Invest. 2016 May 2;126(5):1679-90
pubmed: 27018592
Mech Dev. 2001 Oct;108(1-2):29-43
pubmed: 11578859
Elife. 2019 Apr 01;8:
pubmed: 30932814
Glia. 2020 Jul;68(7):1531-1545
pubmed: 32212285
Sci Rep. 2017 Jun 13;7(1):3375
pubmed: 28611395
Molecules. 2019 Mar 01;24(5):
pubmed: 30832224
Physiol Rev. 2017 Jul 1;97(3):889-938
pubmed: 28468832
Cell Mol Life Sci. 2021 May;78(9):4377-4398
pubmed: 33688979
Lab Anim. 2020 Jun;54(3):213-224
pubmed: 31510859