Natural Occurring Polymorphisms in HIV-1 Integrase and RNase H Regulate Viral Release and Autoprocessing.
RNase H
SNPs
autoprocessing
human immunodeficiency virus
integrase
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
09 11 2021
09 11 2021
Historique:
pubmed:
16
9
2021
medline:
29
12
2021
entrez:
15
9
2021
Statut:
ppublish
Résumé
Recently, a genome-wide association study using plasma HIV RNA from antiretroviral therapy-naive patients reported that 14 naturally occurring nonsynonymous single-nucleotide polymorphisms (SNPs) in HIV derived from antiretrovirus drug-naive patients were associated with virus load (VL). Those SNPs were detected in reverse transcriptase, RNase H, integrase, envelope, and Nef. However, the impact of each mutation on viral fitness was not investigated. Here, we constructed a series of HIV variants encoding each SNP and examined their replicative abilities. An HIV variant containing a Met-to-Ile change at codon 50 in integrase [HIV(IN:M50I)] was found as an impaired virus. Despite the mutation being in integrase, the virus release was significantly suppressed (
Identifiants
pubmed: 34523971
doi: 10.1128/JVI.01323-21
pmc: PMC8577372
doi:
Substances chimiques
Anti-Retroviral Agents
0
Gene Products, gag
0
HIV Integrase
EC 2.7.7.-
Ribonuclease H
EC 3.1.26.4
p31 integrase protein, Human immunodeficiency virus 1
YY6481J2FF
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0132321Subventions
Organisme : CCR NIH HHS
ID : HHSN261200800001C
Pays : United States
Organisme : NCI NIH HHS
ID : HHSN261200800001E
Pays : United States
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