Authentication of a novel antibody to zebrafish collagen type XI alpha 1 chain (Col11a1a).
Antibody authentication
Cartilage
Col11a1a
Collagen α1(XI)
Immunoblot
Immunofluorescence microscopy
Zebrafish
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
15 Sep 2021
15 Sep 2021
Historique:
received:
11
04
2021
accepted:
01
09
2021
entrez:
16
9
2021
pubmed:
17
9
2021
medline:
18
9
2021
Statut:
epublish
Résumé
Extracellular matrix proteins play important roles in embryonic development and antibodies that specifically detect these proteins are essential to understanding their function. The zebrafish embryo is a popular model for vertebrate development but suffers from a dearth of authenticated antibody reagents for research. Here, we describe a novel antibody designed to detect the minor fibrillar collagen chain Col11a1a in zebrafish (AB strain). The Col11a1a antibody was raised in rabbit against a peptide comprising a unique sequence within the zebrafish Col11a1a gene product. The antibody was affinity-purified and characterized by ELISA. The antibody is effective for immunoblot and immunohistochemistry applications. Protein bands identified by immunoblot were confirmed by mass spectrometry and sensitivity to collagenase. Col11a1a knockout zebrafish were used to confirm specificity of the antibody. The Col11a1a antibody labeled cartilaginous structures within the developing jaw, consistent with previously characterized Col11a1 antibodies in other species. Col11a1a within formalin-fixed paraffin-embedded zebrafish were recognized by the antibody. The antibodies and the approaches described here will help to address the lack of well-defined antibody reagents in zebrafish research.
Identifiants
pubmed: 34526111
doi: 10.1186/s13104-021-05770-x
pii: 10.1186/s13104-021-05770-x
pmc: PMC8444443
doi:
Substances chimiques
Antibodies
0
Collagen Type XI
0
Extracellular Matrix Proteins
0
Peptides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
359Subventions
Organisme : NIH HHS
ID : R15HD059949
Pays : United States
Organisme : National Science Foundation
ID : 0923535
Organisme : National Science Foundation
ID : 0619793
Organisme : NIH HHS
ID : K02AR048672
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM109095
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20GM109095
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR047985
Pays : United States
Organisme : NICHD NIH HHS
ID : R15 HD059949
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20GM103408
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM103408
Pays : United States
Organisme : NIH HHS
ID : R01AR047985
Pays : United States
Informations de copyright
© 2021. The Author(s).
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