Long noncoding RNA and protein abundance in lncRNPs.
abundance
concentration
lncRNA
lncRNP
single cell
stoichiometry
subcellular
suborganelle
Journal
RNA (New York, N.Y.)
ISSN: 1469-9001
Titre abrégé: RNA
Pays: United States
ID NLM: 9509184
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
pubmed:
17
9
2021
medline:
15
12
2021
entrez:
16
9
2021
Statut:
ppublish
Résumé
Although long noncoding RNAs (lncRNAs) are generally expressed at low levels, emerging evidence has revealed that many play important roles in gene regulation by a variety of mechanisms as they engage with proteins. Given that the abundance of proteins often greatly exceeds that of their interacting lncRNAs, quantification of the relative abundance, or even the exact stoichiometry in some cases, within lncRNA-protein complexes is helpful for understanding of the mechanism(s) of action of lncRNAs. We discuss methods used to examine lncRNA and protein expression at the single cell, subcellular, and suborganelle levels, the average and local lncRNA concentration in cells, as well as how lncRNAs can modulate the functions of their interacting proteins even at a low stoichiometric concentration.
Identifiants
pubmed: 34526358
pii: rna.078971.121
doi: 10.1261/rna.078971.121
pmc: PMC8594465
doi:
Substances chimiques
RNA, Long Noncoding
0
RNA-Binding Proteins
0
Ribonucleoproteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
1427-1440Subventions
Organisme : Howard Hughes Medical Institute
ID : 55008728
Pays : United States
Informations de copyright
© 2021 Wu et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
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