Use of human PBMC to analyse the impact of obesity on lipid metabolism and metabolic status: a proof-of-concept pilot study.
Absorptiometry, Photon
Adolescent
Adult
Biomarkers
/ blood
Body Composition
Body Weights and Measures
Disease Susceptibility
Female
Humans
Leukocytes, Mononuclear
/ metabolism
Lipid Metabolism
Male
Middle Aged
Obesity
/ etiology
Overweight
/ etiology
Pilot Projects
Principal Component Analysis
Young Adult
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 09 2021
15 09 2021
Historique:
received:
29
03
2021
accepted:
18
08
2021
entrez:
16
9
2021
pubmed:
17
9
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Peripheral blood mononuclear cells (PBMC) are widely used as a biomarker source in nutrition/obesity studies because they reflect gene expression profiles of internal tissues. In this pilot proof-of-concept study we analysed in humans if, as we previously suggested in rodents, PBMC could be a surrogate tissue to study overweight/obesity impact on lipid metabolism. Pre-selected key lipid metabolism genes based in our previous preclinical studies were analysed in PBMC of normoglycemic normal-weight (NW), and overweight-obese (OW-OB) subjects before and after a 6-month weight-loss plan. PBMC mRNA levels of CPT1A, FASN and SREBP-1c increased in the OW-OB group, according with what described in liver and adipose tissue of humans with obesity. This altered expression pattern was related to increased adiposity and early signs of metabolic impairment. Greater weight loss and/or metabolic improvement as result of the intervention was related to lower CPT1A, FASN and SREBP-1c gene expression in an adjusted linear mixed-effects regression analysis, although no gene expression recovery was observed when considering mean comparisons. Thus, human PBMC reflect lipid metabolism expression profile of energy homeostatic tissues, and early obesity-related alterations in metabolic at-risk subjects. Further studies are needed to understand PBMC usefulness for analysis of metabolic recovery in weigh management programs.
Identifiants
pubmed: 34526523
doi: 10.1038/s41598-021-96981-6
pii: 10.1038/s41598-021-96981-6
pmc: PMC8443582
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18329Informations de copyright
© 2021. The Author(s).
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