X-linked immunodeficient (XID) mice exhibit high susceptibility to Cryptococcus gattii infection.
Animals
Biomarkers
Colony Count, Microbial
Cryptococcosis
/ etiology
Cryptococcus gattii
/ immunology
Cytokines
/ metabolism
Disease Models, Animal
Disease Susceptibility
/ immunology
Female
Immunocompromised Host
Immunoglobulin G
/ blood
Immunoglobulin M
/ blood
Mice
X-Linked Combined Immunodeficiency Diseases
/ complications
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 09 2021
15 09 2021
Historique:
received:
13
03
2021
accepted:
19
08
2021
entrez:
16
9
2021
pubmed:
17
9
2021
medline:
17
12
2021
Statut:
epublish
Résumé
Cryptococcosis is an opportunistic disease caused by the fungus Cryptococcus neoformans and Cryptococcus gattii. It starts as a pulmonary infection that can spread to other organs, such as the brain, leading to the most serious occurrence of the disease, meningoencephalitis. The humoral response has already been described in limiting the progression of cryptococcosis where the B-1 cell seems to be responsible for producing natural IgM antibodies, crucial for combating fungal infections. The role of the B-1 cell in C. neoformans infection has been initially described, however the role of the humoral response of B-1 cells has not yet been evaluated during C. gattii infections. In the present study we tried to unravel this issue using XID mice, a murine model deficient in the Btk protein which compromises the development of B-1 lymphocytes. We use the XID mice compared to BALB/c mice that are sufficient for the B-1 population during C. gattii infection. Our model of chronic lung infection revealed that XID mice, unlike the sufficient group of B-1, had early mortality with significant weight loss, in addition to reduced levels of IgM and IgG specific to GXM isolated from the capsule of C. neoformans. In addition to this, we observed an increased fungal load in the blood and in the brain. We described an increase in the capsular size of C. gattii and the predominant presence of cytokines with a Th2 profile was also observed in these animals. Thus, the present study strongly points to a higher susceptibility of the XID mouse to C. gattii, which suggests that the presence of B-1 cells and anti-GXM antibodies is fundamental during the control of infection by C. gattii.
Identifiants
pubmed: 34526536
doi: 10.1038/s41598-021-97041-9
pii: 10.1038/s41598-021-97041-9
pmc: PMC8443669
doi:
Substances chimiques
Biomarkers
0
Cytokines
0
Immunoglobulin G
0
Immunoglobulin M
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18397Informations de copyright
© 2021. The Author(s).
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