EndoBind detects endogenous protein-protein interactions in real time.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
15 09 2021
15 09 2021
Historique:
received:
26
01
2021
accepted:
24
08
2021
entrez:
16
9
2021
pubmed:
17
9
2021
medline:
15
12
2021
Statut:
epublish
Résumé
We present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer formation over an extended duration. Using examples of the Nrf2-KEAP1 and the CRAF-KRAS-G12V interaction, we demonstrate that our method allows for the detection of signal for more than 2 days after substrate addition, allowing for continuous monitoring of endogenous protein-protein interactions in real time.
Identifiants
pubmed: 34526658
doi: 10.1038/s42003-021-02600-5
pii: 10.1038/s42003-021-02600-5
pmc: PMC8443649
doi:
Substances chimiques
KEAP1 protein, human
0
KRAS protein, human
0
Kelch-Like ECH-Associated Protein 1
0
NF-E2-Related Factor 2
0
NFE2L2 protein, human
0
Proto-Oncogene Proteins p21(ras)
EC 3.6.5.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1085Informations de copyright
© 2021. The Author(s).
Références
Chem Res Toxicol. 2005 Dec;18(12):1917-26
pubmed: 16359182
J Biol Chem. 2016 Nov 18;291(47):24579-24593
pubmed: 27703006
J Med Chem. 2016 Apr 28;59(8):3991-4006
pubmed: 27031670
Chem Biol. 2006 Feb;13(2):213-23
pubmed: 16492569
Free Radic Biol Med. 2015 Nov;88(Pt B):101-107
pubmed: 26057936
Cancer Cell. 2016 Sep 12;30(3):485-498
pubmed: 27523909
ChemMedChem. 2016 Apr 19;11(8):738-56
pubmed: 26864455
J Allergy Clin Immunol. 2019 Jul;144(1):224-235
pubmed: 30707971
Nat Rev Drug Discov. 2020 Aug;19(8):533-552
pubmed: 32528145
Nat Rev Cancer. 2017 Nov;17(11):676-691
pubmed: 28984291
J Biol Chem. 2013 Jan 25;288(4):2403-13
pubmed: 23188824
Biochem Pharmacol. 2018 Feb;148:298-307
pubmed: 29309765
Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):14746-53
pubmed: 22908297
Nat Med. 2005 Sep;11(9):920-2
pubmed: 16145569
ACS Chem Biol. 2016 Feb 19;11(2):400-8
pubmed: 26569370
FASEB J. 2019 Mar;33(3):3841-3850
pubmed: 30521377
Clin Cancer Res. 2007 Mar 1;13(5):1576-83
pubmed: 17332304
Breast Cancer Res. 2008;10(2):R33
pubmed: 18430202
Anal Biochem. 2017 Apr 1;522:10-17
pubmed: 28115169
Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):57-62
pubmed: 16371460