Comparison of stem cells from human exfoliated deciduous posterior teeth with varying levels of root resorption.
Stem cells from human exfoliated deciduous posterior teeth
differentiation
in vitro culture
markers expression
root resorption
Journal
Stem cell investigation
ISSN: 2306-9759
Titre abrégé: Stem Cell Investig
Pays: China
ID NLM: 101672113
Informations de publication
Date de publication:
2021
2021
Historique:
received:
26
08
2020
accepted:
17
05
2021
entrez:
16
9
2021
pubmed:
17
9
2021
medline:
17
9
2021
Statut:
epublish
Résumé
Stem cells from human exfoliated deciduous teeth (SHED) are regarded as an attractive cell source for tissue regeneration. However, the effect of different levels of root resorption on the characteristics of SHED remains less understood. Thus, the tooth source that is most suitable for the isolation of SHEDs needs to be determined. To compare cellular and biological characteristics of stem cells from human exfoliated deciduous posterior teeth with varying levels of root resorption. The pulp was obtained from the deciduous posterior teeth depending on the level of root resorption, and isolated SHEDs were grouped as follows: Teeth with 0 to 1/3 No major differences were observed in terms of cellular morphology, viability, proliferation rate, colony-forming ability, cell surface markers expression, and mesenchymal lineage differentiation of SHEDs isolated from posterior teeth with 0 to 1/3 Collectively, SHEDs (G1) and SHEDs (G2) showed comparable cellular and biological characteristics that enable their possible applications in regenerative therapies.
Sections du résumé
BACKGROUND
BACKGROUND
Stem cells from human exfoliated deciduous teeth (SHED) are regarded as an attractive cell source for tissue regeneration. However, the effect of different levels of root resorption on the characteristics of SHED remains less understood. Thus, the tooth source that is most suitable for the isolation of SHEDs needs to be determined. To compare cellular and biological characteristics of stem cells from human exfoliated deciduous posterior teeth with varying levels of root resorption.
METHODS
METHODS
The pulp was obtained from the deciduous posterior teeth depending on the level of root resorption, and isolated SHEDs were grouped as follows: Teeth with 0 to 1/3
RESULTS
RESULTS
No major differences were observed in terms of cellular morphology, viability, proliferation rate, colony-forming ability, cell surface markers expression, and mesenchymal lineage differentiation of SHEDs isolated from posterior teeth with 0 to 1/3
CONCLUSIONS
CONCLUSIONS
Collectively, SHEDs (G1) and SHEDs (G2) showed comparable cellular and biological characteristics that enable their possible applications in regenerative therapies.
Identifiants
pubmed: 34527730
doi: 10.21037/sci-2020-039
pii: sci-08-2020-039
pmc: PMC8413134
doi:
Types de publication
Journal Article
Langues
eng
Pagination
15Informations de copyright
2021 Stem Cell Investigation. All rights reserved.
Déclaration de conflit d'intérêts
Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at http://dx.doi.org/10.21037/sci-2020-039). The authors have no conflicts of interest to declare.
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