Retinal Degeneration Caused by Ago2 Disruption.

Animals Argonaute Proteins / biosynthesis Disease Models, Animal Electroretinography Gene Expression Regulation Mice, Inbred C57BL MicroRNAs / genetics Retina Retinal Degeneration / diagnosis Tomography, Optical Coherence / methods

Journal

Investigative ophthalmology & visual science
ISSN: 1552-5783
Titre abrégé: Invest Ophthalmol Vis Sci
Pays: United States
ID NLM: 7703701

Informations de publication

Date de publication:
02 09 2021
Historique:
entrez: 16 9 2021
pubmed: 17 9 2021
medline: 29 9 2021
Statut: ppublish

Résumé

Argonaute proteins are key players in small RNA-guided gene silencing processes. Ago2 is the member of the Argonaute subfamily with slicer endonuclease activity and is critical for microRNA homeostasis and indispensable for biological development. However, the impact of Ago2 dysregulation in the retina remains to be fully explored. In this study, we studied the role of Ago2 in mouse retina. We explored the function of Ago2 in the mouse retina through an adeno-associated virus-mediated Ago2 disruption mouse model. An ERG was carried out to determine the retinal function. Spectral domain optical coherence tomography, fundus photographs, and immunostaining were performed to investigate the retinal structure. A quantitative RT-PCR assay was used to determine the expression of noncoding RNAs. Both silencing and overexpression of Ago2 in mouse retina resulted in significant retinal morphological alterations and severe impairment of retinal function, mainly with a thinned outer nuclear layer, shortened inner segment/outer segment, and diminished ERG responses. Furthermore, Ago2 disruption resulted in alterations of noncoding RNAs in retina. Our finding demonstrated that Ago2 interruption led to severe retinal degeneration, suggested that Ago2 homeostasis contributed to retinal structural and functional maintenance.

Identifiants

DOI: 10.1167/iovs.62.12.14 PMID: 34529004 PMC: PMC8447045
pubmed: 34529004
pii: 2777905
doi: 10.1167/iovs.62.12.14
pmc: PMC8447045
doi:

Substances chimiques

Ago2 protein, mouse 0
Argonaute Proteins 0
MicroRNAs 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

14

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Auteurs

Xue-Jiao Chen (XJ)

School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, China.

Chang-Jun Zhang (CJ)

Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory, Beijing, China.

Ya-Han Wang (YH)

School of Ophthalmology & Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, China.
Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory, Beijing, China.

Zi-Bing Jin (ZB)

Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory, Beijing, China.
School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, China.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Retinal Degeneration Caused by Ago2 Disruption.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Ribonucléoprotéines › Complexe réprimant l'expression de l'ARN › Protéines Argonaute › Retinal Degeneration Caused by Ago2 Disruption.
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Retinal Degeneration Caused by Ago2 Disruption.
questionsmedicales.fr › Diagnostic › Techniques et procédures diagnostiques › Électrodiagnostic › Électrorétinographie › Retinal Degeneration Caused by Ago2 Disruption.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Retinal Degeneration Caused by Ago2 Disruption.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › Retinal Degeneration Caused by Ago2 Disruption.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Retinal Degeneration Caused by Ago2 Disruption.
questionsmedicales.fr › Organes des sens › Oeil › Rétine › Retinal Degeneration Caused by Ago2 Disruption.
questionsmedicales.fr › Maladies de l'oeil › Rétinopathies › Dégénérescence de la rétine › Retinal Degeneration Caused by Ago2 Disruption.
questionsmedicales.fr › Techniques d'investigation › Imagerie optique › Tomographie optique › Tomographie par cohérence optique › Retinal Degeneration Caused by Ago2 Disruption.