Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
23
06
2021
accepted:
31
08
2021
entrez:
16
9
2021
pubmed:
17
9
2021
medline:
24
11
2021
Statut:
epublish
Résumé
With the advance of precision medicine, the availability of tumor tissue for molecular analysis has become a limiting factor. This is particularly the case for bone metastases which are frequently occurring in cancer types such as prostate cancer. Due to the necessary decalcification process it was long thought that transcriptome analysis will not be feasible from decalcified formalin-fixed, paraffin-embedded (DFFPE) in a large manner. Here we demonstrate that mRNA extraction from DFFPE is feasible, quick, robust and reproducible and that decalcification does not hamper subsequent gene expression analysis. This might assist in implementing transcriptome analysis from DFFPE into every day practice.
Identifiants
pubmed: 34529723
doi: 10.1371/journal.pone.0257416
pii: PONE-D-21-18567
pmc: PMC8445430
doi:
Substances chimiques
RNA, Messenger
0
Formaldehyde
1HG84L3525
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0257416Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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