Cooperation between melanoma cell states promotes metastasis through heterotypic cluster formation.
TFAP2
circulating tumor cell cluster
cluster
cooperation
melanoma
metastasis
zebrafish
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
25 10 2021
25 10 2021
Historique:
received:
13
10
2020
revised:
07
07
2021
accepted:
20
08
2021
pubmed:
17
9
2021
medline:
16
12
2021
entrez:
16
9
2021
Statut:
ppublish
Résumé
Melanomas can have multiple coexisting cell states, including proliferative (PRO) versus invasive (INV) subpopulations that represent a "go or grow" trade-off; however, how these populations interact is poorly understood. Using a combination of zebrafish modeling and analysis of patient samples, we show that INV and PRO cells form spatially structured heterotypic clusters and cooperate in the seeding of metastasis, maintaining cell state heterogeneity. INV cells adhere tightly to each other and form clusters with a rim of PRO cells. Intravital imaging demonstrated cooperation in which INV cells facilitate dissemination of less metastatic PRO cells. We identified the TFAP2 neural crest transcription factor as a master regulator of clustering and PRO/INV states. Isolation of clusters from patients with metastatic melanoma revealed a subset with heterotypic PRO-INV clusters. Our data suggest a framework for the co-existence of these two divergent cell populations, in which heterotypic clusters promote metastasis via cell-cell cooperation.
Identifiants
pubmed: 34529939
pii: S1534-5807(21)00677-8
doi: 10.1016/j.devcel.2021.08.018
pmc: PMC8551056
mid: NIHMS1737985
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2808-2825.e10Subventions
Organisme : NCI NIH HHS
ID : U54 CA137788
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA083115
Pays : United States
Organisme : Cancer Research UK
ID : C8216/A6129
Pays : United Kingdom
Organisme : NCI NIH HHS
ID : F30 CA220954
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : Medical Research Council
ID : MR/M019012/1
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C588/A19167
Pays : United Kingdom
Organisme : NCI NIH HHS
ID : K00 CA223016
Pays : United States
Organisme : Cancer Research UK
ID : 8466
Pays : United Kingdom
Organisme : NIAMS NIH HHS
ID : R01 AR062547
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA229215
Pays : United States
Organisme : NCI NIH HHS
ID : DP2 CA186572
Pays : United States
Organisme : Cancer Research UK
ID : C7535/A6408
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C2195/A8466
Pays : United Kingdom
Organisme : NCI NIH HHS
ID : U54 CA132378
Pays : United States
Organisme : Cancer Research UK
ID : C588/A10721
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/V000292/1
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : T32 GM007739
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests M.R.M. receives research funding from GRAIL. D.J.A. is a paid consultant for Microbiotica and receives researching funding from Astra Zeneca and OpenTargets. M.P.L. receives research funding from Roche and Novartis. N.A. is a paid consultant for companies with an interest in liquid biopsy. R.M.W. is a paid consultant to N-of-One Therapeutics, a subsidiary of Qiagen. R.M.W. is on the Scientific Advisory Board of Consano but receives no income for this. R.M.W. receives royalty payments for the use of the casper line from Carolina Biologicals.
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