Designing libraries for pooled CRISPR functional screens of long noncoding RNAs.
Journal
Mammalian genome : official journal of the International Mammalian Genome Society
ISSN: 1432-1777
Titre abrégé: Mamm Genome
Pays: United States
ID NLM: 9100916
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
07
07
2021
accepted:
09
09
2021
pubmed:
18
9
2021
medline:
20
5
2022
entrez:
17
9
2021
Statut:
ppublish
Résumé
Human and other genomes encode tens of thousands of long noncoding RNAs (lncRNAs), the vast majority of which remain uncharacterised. High-throughput functional screening methods, notably those based on pooled CRISPR-Cas perturbations, promise to unlock the biological significance and biomedical potential of lncRNAs. Such screens are based on libraries of single guide RNAs (sgRNAs) whose design is critical for success. Few off-the-shelf libraries are presently available, and lncRNAs tend to have cell-type-specific expression profiles, meaning that library design remains in the hands of researchers. Here we introduce the topic of pooled CRISPR screens for lncRNAs and guide readers through the three key steps of library design: accurate annotation of transcript structures, curation of optimal candidate sets, and design of sgRNAs. This review is a starting point and reference for researchers seeking to design custom CRISPR screening libraries for lncRNAs.
Identifiants
pubmed: 34533605
doi: 10.1007/s00335-021-09918-9
pii: 10.1007/s00335-021-09918-9
pmc: PMC9114037
doi:
Substances chimiques
RNA, Guide
0
RNA, Long Noncoding
0
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
312-327Informations de copyright
© 2021. The Author(s).
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