Cell patterning by secretion-induced plasma membrane flows.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
17 Sep 2021
17 Sep 2021
Historique:
entrez:
17
9
2021
pubmed:
18
9
2021
medline:
18
9
2021
Statut:
ppublish
Résumé
Cells self-organize using reaction-diffusion and fluid-flow principles. Whether bulk membrane flows contribute to cell patterning has not been established. Here, using mathematical modeling, optogenetics, and synthetic probes, we show that polarized exocytosis causes lateral membrane flows away from regions of membrane insertion. Plasma membrane–associated proteins with sufficiently low diffusion and/or detachment rates couple to the flows and deplete from areas of exocytosis. In rod-shaped fission yeast cells, zones of Cdc42 GTPase activity driving polarized exocytosis are limited by GTPase activating proteins (GAPs). We show that membrane flows pattern the GAP Rga4 distribution and that coupling of a synthetic GAP to membrane flows is sufficient to establish the rod shape. Thus, membrane flows induced by Cdc42-dependent exocytosis form a negative feedback restricting the zone of Cdc42 activity.
Identifiants
pubmed: 34533984
doi: 10.1126/sciadv.abg6718
pmc: PMC8448446
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
eabg6718Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM114201
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136372
Pays : United States
Références
Curr Opin Cell Biol. 2020 Feb;62:123-134
pubmed: 31760155
Annu Rev Cell Dev Biol. 2012;28:215-50
pubmed: 22905956
Curr Biol. 1999 Dec 2;9(23):1411-4
pubmed: 10607569
Cells. 2020 Apr 30;9(5):
pubmed: 32365827
PLoS Biol. 2020 Jan 24;18(1):e3000600
pubmed: 31978045
Curr Biol. 2006 Dec 19;16(24):2480-7
pubmed: 17140794
Phys Biol. 2010 Jun 04;7(2):026011
pubmed: 20526029
Mol Biol Cell. 2013 Jun;24(12):1872-81
pubmed: 23615447
Cells. 2020 Jun 23;9(6):
pubmed: 32585819
Mol Biol Cell. 2007 Jun;18(6):2090-101
pubmed: 17377067
Curr Biol. 2003 Sep 16;13(18):1636-40
pubmed: 13678596
PLoS Biol. 2015 Apr 02;13(4):e1002097
pubmed: 25837586
PLoS One. 2012;7(6):e40248
pubmed: 22768263
Methods. 2017 Feb 15;115:80-90
pubmed: 27713081
Elife. 2021 May 13;10:
pubmed: 33983119
Yeast. 1998 Jul;14(10):943-51
pubmed: 9717240
Eukaryot Cell. 2015 Oct;14(10):1017-42
pubmed: 26253157
Mol Biol Cell. 2001 Apr;12(4):1061-77
pubmed: 11294907
Mol Biol Cell. 2002 Aug;13(8):2963-76
pubmed: 12181359
Phys Biol. 2004 Dec;1(3-4):137-51
pubmed: 16204833
Elife. 2019 May 03;8:
pubmed: 31050340
J Cell Biol. 2009 Jan 26;184(2):297-308
pubmed: 19153221
Phys Rev E Stat Nonlin Soft Matter Phys. 2014 Feb;89(2):022724
pubmed: 25353525
J Cell Biol. 2010 May 3;189(3):399-406
pubmed: 20439993
Cell. 2012 Aug 3;150(3):508-20
pubmed: 22863005
J Cell Biol. 2020 Jun 1;219(6):
pubmed: 32320462
PLoS Comput Biol. 2018 Jul 20;14(7):e1006317
pubmed: 30028833
Dev Cell. 2018 Jul 2;46(1):9-22.e4
pubmed: 29937389
Comput Biol Chem. 2021 Apr;91:107437
pubmed: 33517146
Cell Rep. 2020 Jan 14;30(2):421-431.e7
pubmed: 31940486
Dev Cell. 2013 Jul 29;26(2):148-61
pubmed: 23906065
PLoS One. 2014 Jan 17;9(1):e84524
pubmed: 24465414
Front Plant Sci. 2017 Feb 07;8:154
pubmed: 28224002
Curr Biol. 2016 Nov 7;26(21):2921-2928
pubmed: 27746023
Mol Biol Cell. 2011 Jan 1;22(1):44-53
pubmed: 21148300
Curr Biol. 2011 Feb 8;21(3):184-94
pubmed: 21277209
Soft Matter. 2016 Feb 21;12(7):2127-34
pubmed: 26751985
Bioessays. 2020 Jan;42(1):e1900142
pubmed: 31823398
J Cell Biol. 2015 Mar 30;208(7):897-911
pubmed: 25825517
Nat Commun. 2017 Sep 15;8(1):547
pubmed: 28916751
J Chem Phys. 2014 Jun 7;140(21):214115
pubmed: 24907998
Fungal Genet Biol. 2010 Jul;47(7):573-86
pubmed: 20302965
Nat Methods. 2013 Mar;10(3):249-52
pubmed: 23377377
J Cell Biol. 2013 Feb 18;200(4):407-18
pubmed: 23401000
Nat Struct Mol Biol. 2008 Jul;15(7):675-83
pubmed: 18596814
Biophys J. 2001 Jun;80(6):2667-77
pubmed: 11371443
Dev Cell. 2014 Mar 10;28(5):534-46
pubmed: 24636258
Mol Biol Cell. 2008 Apr;19(4):1439-49
pubmed: 18216285
Biophys J. 2010 Nov 3;99(9):2879-87
pubmed: 21044585
Nature. 2009 Jun 11;459(7248):852-6
pubmed: 19474792
J Chem Phys. 2019 Sep 28;151(12):124104
pubmed: 31575184
Dev Cell. 2015 Nov 23;35(4):471-82
pubmed: 26609960
ACS Synth Biol. 2020 Apr 17;9(4):902-919
pubmed: 32155337
Cell. 2018 Dec 13;175(7):1769-1779.e13
pubmed: 30392960
Curr Biol. 2008 Mar 11;18(5):322-30
pubmed: 18328707
Biophys J. 1999 Jan;76(1 Pt 1):314-22
pubmed: 9876143
Mol Biol Cell. 2011 Oct;22(20):3801-11
pubmed: 21849474
J Chem Phys. 2015 Aug 28;143(8):084117
pubmed: 26328828
Mol Biol Cell. 2002 Feb;13(2):515-29
pubmed: 11854409
PLoS Biol. 2019 Jun 24;17(6):e3000060
pubmed: 31233488
Sci Rep. 2017 Oct 11;7(1):12970
pubmed: 29021607
Cell. 1995 Dec 1;83(5):693-701
pubmed: 8521486
Biochemistry. 2012 Feb 28;51(8):1638-47
pubmed: 22263647
Biophys J. 2011 Mar 2;100(5):1261-70
pubmed: 21354399
PLoS Biol. 2016 Apr 15;14(4):e1002437
pubmed: 27082518
Mol Biol Cell. 2010 Aug 15;21(16):2894-904
pubmed: 20587778
Mol Biol Cell. 2016 Mar 9;:
pubmed: 26960792
J Cell Biol. 2020 Nov 2;219(11):
pubmed: 33053166
Biophys Rev. 2018 Dec;10(6):1649-1666
pubmed: 30448943
Biophys J. 2017 Jul 11;113(1):132-137
pubmed: 28700911
Curr Biol. 2008 Nov 25;18(22):1719-26
pubmed: 19013066
Mol Biol Cell. 2012 Sep;23(17):3336-47
pubmed: 22767578
Science. 1984 May 18;224(4650):681-6
pubmed: 6719108
Biophys J. 2017 Apr 25;112(8):1682-1691
pubmed: 28445759
J Cell Biol. 2018 Dec 3;217(12):4215-4229
pubmed: 30279276
J Biol Chem. 2010 Feb 19;285(8):5738-47
pubmed: 20018884
J Cell Biol. 2012 Nov 26;199(5):831-47
pubmed: 23185032
J Biol Chem. 1994 Sep 30;269(39):24229-36
pubmed: 7929079
Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9045-50
pubmed: 16754851
J Cell Sci. 2020 Jan 8;133(1):
pubmed: 31801797
Microbiol Spectr. 2017 Apr;5(2):
pubmed: 28429675
Mol Cell Biol. 2013 Jan;33(2):418-28
pubmed: 23149940
Cell. 2012 Apr 13;149(2):322-33
pubmed: 22500799
Nature. 2009 Jun 11;459(7248):857-60
pubmed: 19474789
Science. 2012 Jul 13;337(6091):239-43
pubmed: 22604726
J Cell Sci. 2008 Dec 1;121(Pt 23):3878-89
pubmed: 18984630
Cell. 2011 Jun 24;145(7):1116-28
pubmed: 21703453