Fluorophore-PNA-Quencher/Quencher-DNA probe for miRNA detection.
Micro RNA
PNA/DNA hybrid probe
Peptide nucleic acid
RNA detection
Journal
Bioorganic & medicinal chemistry letters
ISSN: 1464-3405
Titre abrégé: Bioorg Med Chem Lett
Pays: England
ID NLM: 9107377
Informations de publication
Date de publication:
01 11 2021
01 11 2021
Historique:
received:
06
07
2021
revised:
26
08
2021
accepted:
09
09
2021
pubmed:
18
9
2021
medline:
11
1
2022
entrez:
17
9
2021
Statut:
ppublish
Résumé
Micro RNAs (miRNAs) are involved in a variety of biological functions and are attracting attention as diagnostic and prognostic markers for various diseases. Highly sensitive RNA detection methods are required to determine miRNA expression levels and intracellular localization. In this study, we designed new double-stranded peptide nucleic acid (PNA)/DNA probes consisting of a fluorophore-PNA-quencher (fPq) and a quencher-DNA (qD) for miR-221 detection. We optimized the fPq structure, PNA-DNA hybrid length, and hybrid position. The resultant fPq-2/qD-6b probe was a 6-bp hybrid probe with a 10-base fPq and a 6-base qD. The signal-to-background ratios of the probes showed that fPq-2/qD-6b had a higher target sensitivity than fPq (PNA beacon)-type and fP/qD-type probes. The results of the detection limit and target specificity indicate that the fPq/qD probe is promising for RNA detection in both cells and cell extracts as well as for miRNA diagnosis.
Identifiants
pubmed: 34534675
pii: S0960-894X(21)00586-2
doi: 10.1016/j.bmcl.2021.128359
pii:
doi:
Substances chimiques
DNA Probes
0
Fluorescent Dyes
0
MicroRNAs
0
Peptide Nucleic Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
128359Informations de copyright
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