A Single-cell Transcriptome Atlas of Cashmere Goat Hair Follicle Morphogenesis.
Cashmere goat
Cellular heterogeneity
Developmental trajectory
Hair follicle morphogenesis
Single-cell transcriptome
Journal
Genomics, proteomics & bioinformatics
ISSN: 2210-3244
Titre abrégé: Genomics Proteomics Bioinformatics
Pays: China
ID NLM: 101197608
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
08
06
2020
revised:
17
06
2021
accepted:
02
08
2021
pubmed:
18
9
2021
medline:
24
3
2022
entrez:
17
9
2021
Statut:
ppublish
Résumé
Cashmere, also known as soft gold, is produced from the secondary hair follicles (SHFs) of cashmere goats. The number of SHFs determines the yield and quality of cashmere; therefore, it is of interest to investigate the transcriptional profiles present during cashmere goat hair follicle development. However, mechanisms underlying this development process remain largely unexplored, and studies regarding hair follicle development mostly use a murine research model. In this study, to provide a comprehensive understanding of cellular heterogeneity and cell fate decisions, single-cell RNA sequencing was performed on 19,705 single cells of the dorsal skin from cashmere goat fetuses at induction (embryonic day 60; E60), organogenesis (E90), and cytodifferentiation (E120) stages. For the first time, unsupervised clustering analysis identified 16 cell clusters, and their corresponding cell types were also characterized. Based on lineage inference, a detailed molecular landscape was revealed along the dermal and epidermal cell lineage developmental pathways. Notably, our current data also confirmed the heterogeneity of dermal papillae from different hair follicle types, which was further validated by immunofluorescence analysis. The current study identifies different biomarkers during cashmere goat hair follicle development and has implications for cashmere goat breeding in the future.
Identifiants
pubmed: 34534715
pii: S1672-0229(21)00179-0
doi: 10.1016/j.gpb.2021.07.003
pmc: PMC8864196
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
437-451Informations de copyright
Copyright © 2021 The Author. Published by Elsevier B.V. All rights reserved.
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