Kruppel-like factor 15 induces the development of mature hepatocyte-like cells from hepatoblasts.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 09 2021
17 09 2021
Historique:
received:
19
05
2021
accepted:
01
09
2021
entrez:
18
9
2021
pubmed:
19
9
2021
medline:
16
11
2021
Statut:
epublish
Résumé
The liver is an important metabolic organ that controls homeostasis in the body. Moreover, it functions as a hematopoietic organ, while its metabolic function is low during development. Hepatocytes, which are parenchymal cells of the liver, acquire various metabolic functions by the maturation of hepatic progenitor cells during the fetal period; however, this molecular mechanism is still unclear. In this study, Kruppel-like factor 15 (KLF15) was identified as a new regulator of hepatic maturation through a comprehensive analysis of the expression of transcriptional regulators in mouse fetal and adult hepatocytes. KLF15 is a transcription factor whose expression in the liver increases from the embryonic stage throughout the developmental process. KLF15 induced the overexpression of liver function genes in mouse embryonic hepatocytes. Furthermore, we found that the expression of KLF15 could also induce the expression of liver function genes in hepatoblasts derived from human induced pluripotent stem cells (iPSCs). Moreover, KLF15 increased the promoter activity of tyrosine aminotransferase, a liver function gene. KLF15 also suppressed the proliferation of hepatoblasts. These results suggest that KLF15 induces hepatic maturation through the transcriptional activation of target genes and cell cycle control.
Identifiants
pubmed: 34535735
doi: 10.1038/s41598-021-97937-6
pii: 10.1038/s41598-021-97937-6
pmc: PMC8448749
doi:
Substances chimiques
KLF15 protein, human
0
Klf15 protein, mouse
0
Kruppel-Like Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18551Informations de copyright
© 2021. The Author(s).
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