Prolyl isomerase Pin1 plays an essential role in SARS-CoV-2 proliferation, indicating its possibility as a novel therapeutic target.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 09 2021
17 09 2021
Historique:
received:
28
02
2021
accepted:
30
08
2021
entrez:
18
9
2021
pubmed:
19
9
2021
medline:
30
9
2021
Statut:
epublish
Résumé
Novel coronavirus disease 2019 (COVID-19) has emerged as a global pandemic with far-reaching societal impact. Here we demonstrate that Pin1 is a key cellular molecule necessary for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) propagation. In this study, siRNA-mediated silencing of Pin1 expression markedly suppressed the proliferation of SARS-CoV-2 in VeroE6/TMPRSS2 cells. In addition, several recently generated Pin1 inhibitors showed strong inhibitory effects on SARS-CoV-2 proliferation, measured by both viral mRNA and protein synthesis, and alleviated the cytopathic effect (CPE) on VeroE6/TMPRSS2 cells. One compound, termed H-77, was found to block SARS-CoV-2 proliferation at an EC
Identifiants
pubmed: 34535740
doi: 10.1038/s41598-021-97972-3
pii: 10.1038/s41598-021-97972-3
pmc: PMC8448864
doi:
Substances chimiques
NIMA-Interacting Peptidylprolyl Isomerase
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18581Subventions
Organisme : Japan Society for the Promotion of Science
ID : Grant-in-Aid for Scientific Research (C)
Organisme : Japan Society for the Promotion of Science
ID : Grant-in-Aid for Scientific Research (C)
Organisme : Japan Society for the Promotion of Science
ID : Grant-in-Aid for Scientific Research (B)
Organisme : Japan Agency for Medical Research and Development
ID : Development of Technology to Control Viral Infections
Organisme : Hiroshima Prefecture, Japan
ID : Goverment-Academia Collaboration
Informations de copyright
© 2021. The Author(s).
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