Supplemental nitrite increases choroidal neovascularization in mice.
Age-related macular degeneration
Aging
Choroidal neovascularization
Nitrate
Nitrite
Journal
Nitric oxide : biology and chemistry
ISSN: 1089-8611
Titre abrégé: Nitric Oxide
Pays: United States
ID NLM: 9709307
Informations de publication
Date de publication:
01 12 2021
01 12 2021
Historique:
received:
24
07
2021
revised:
09
09
2021
accepted:
14
09
2021
pubmed:
20
9
2021
medline:
28
1
2022
entrez:
19
9
2021
Statut:
ppublish
Résumé
Low doses of nitrite, close to physiological levels, increase blood flow in normal and ischemic tissues through a nitric oxide (NO) dependent mechanism. Given that nitrite therapy and dietary supplementation with vegetables high in nitrate (e.g. beets) are gaining popularity we decided to determine if low doses of nitrite impact the development of choroidal neovascularization (CNV), a key feature of wet age related macular degeneration (AMD). Sodium nitrite (at 50 mg/L, 150 mg/L, and 300 mg/L), nitrate (1 g/L) or water alone were provided in the drinking water of C57BL/6 J mice aged 2 or 12 months. Mice were allowed to drink ad libitum for 1 week at which time laser-induced choroidal neovascularization (L-CNV) was induced. The mice continued to drink the supplemented water ad libitum for a further 14 days at which point optical coherence tomography (OCT) was performed to determine the volume of the CNV lesion. Blood was drawn to determine nitrite and nitrate levels and eyes taken for histology. CNV volume was 2.86 × 10
Identifiants
pubmed: 34537345
pii: S1089-8603(21)00093-8
doi: 10.1016/j.niox.2021.09.005
pmc: PMC8647281
mid: NIHMS1742245
pii:
doi:
Substances chimiques
Nitrates
0
Nitrites
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7-15Subventions
Organisme : NEI NIH HHS
ID : P30 EY003039
Pays : United States
Organisme : NIDCR NIH HHS
ID : T90 DE022736
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001417
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR003096
Pays : United States
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
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