Zinc-chelating postsynaptic density-95 N-terminus impairs its palmitoyl modification.
N-terminal domain
PSD-95
palmitoylation
zinc binding
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
revised:
10
09
2021
received:
07
07
2021
accepted:
17
09
2021
pubmed:
20
9
2021
medline:
25
12
2021
entrez:
19
9
2021
Statut:
ppublish
Résumé
Chemical synaptic transmission represents the most sophisticated dynamic process and is highly regulated with optimized neurotransmitter balance. Imbalanced transmitters can lead to transmission impairments, for example, intracellular zinc accumulation is a hallmark of degenerating neurons. However, the underlying mechanisms remain elusive. Postsynaptic density protein-95 (PSD-95) is a primary postsynaptic membrane-associated protein and the major scaffolding component in the excitatory postsynaptic densities, which performs substantial functions in synaptic development and maturation. Its membrane association induced by palmitoylation contributes largely to its regulatory functions at postsynaptic sites. Unlike other structural domains in PSD-95, the N-terminal region (PSD-95NT) is flexible and interacts with various targets, which modulates its palmitoylation of two cysteines (C3/C5) and glutamate receptor distributions in postsynaptic densities. PSD-95NT contains a putative zinc-binding motif (C2H2) with undiscovered functions. This study is the first effort to investigate the interaction between Zn
Identifiants
pubmed: 34538002
doi: 10.1002/pro.4187
pmc: PMC8521293
doi:
Substances chimiques
Chelating Agents
0
DLG4 protein, human
0
Disks Large Homolog 4 Protein
0
Zinc
J41CSQ7QDS
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2246-2257Informations de copyright
© 2021 The Protein Society.
Références
J Neurosci. 2013 Jul 17;33(29):12122-35
pubmed: 23864697
Neuron. 2004 Dec 16;44(6):987-96
pubmed: 15603741
J Cell Sci. 2010 Dec 1;123(Pt 23):4007-10
pubmed: 21084560
Elife. 2019 Mar 13;8:
pubmed: 30864948
Prog Nucl Magn Reson Spectrosc. 2013 Aug;73:1-16
pubmed: 23962882
Sci Adv. 2020 Jul 3;6(27):
pubmed: 32937457
Biomol NMR Assign. 2021 Oct;15(2):347-350
pubmed: 33929702
Brain Res. 1994 Aug 22;654(2):257-64
pubmed: 7527288
Proc Natl Acad Sci U S A. 2016 Aug 9;113(32):E4736-44
pubmed: 27457929
J Biol Chem. 2000 Aug 4;275(31):23904-10
pubmed: 10779526
Protein Sci. 1992 May;1(5):563-74
pubmed: 1304355
J Nutr. 2000 May;130(5S Suppl):1471S-83S
pubmed: 10801962
Front Mol Neurosci. 2019 Jan 31;12:8
pubmed: 30766476
ACS Chem Biol. 2017 Sep 15;12(9):2313-2323
pubmed: 28692247
Proc Natl Acad Sci U S A. 2018 Nov 27;115(48):E11302-E11310
pubmed: 30420502
Neuroscience. 2009 Jan 12;158(1):126-36
pubmed: 18353558
Mol Cell Proteomics. 2007 Oct;6(10):1749-60
pubmed: 17623647
Biophys J. 2011 Apr 20;100(8):1910-8
pubmed: 21504727
Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):E6983-92
pubmed: 26604311
Proc Natl Acad Sci U S A. 2013 May 28;110(22):9118-23
pubmed: 23671101
Nucleic Acids Res. 2003 Jan 15;31(2):532-50
pubmed: 12527760
Nat Rev Neurosci. 2004 Oct;5(10):771-81
pubmed: 15378037
J Neurosci. 2004 Nov 10;24(45):10248-59
pubmed: 15537897
PLoS One. 2013;8(1):e55384
pubmed: 23383172
Methods Enzymol. 2000;326:245-54
pubmed: 11036646
Neuron. 1992 Nov;9(5):929-42
pubmed: 1419001
Neuroscience. 1994 Jun;60(4):1049-57
pubmed: 7936205
J Biol Chem. 2003 May 30;278(22):20225-34
pubmed: 12644454
Mol Brain. 2014 Mar 07;7:16
pubmed: 24602382
Biochemistry. 1994 May 17;33(19):5984-6003
pubmed: 7514039
J Neurophysiol. 2006 Mar;95(3):1949-56
pubmed: 16319204
Front Synaptic Neurosci. 2019 Mar 12;11:6
pubmed: 30914943
EMBO J. 2014 Jun 17;33(12):1341-53
pubmed: 24705785
PLoS One. 2012;7(9):e46012
pubmed: 23049922
Proc Natl Acad Sci U S A. 2016 Dec 27;113(52):E8482-E8491
pubmed: 27956638
Curr Opin Neurobiol. 2011 Apr;21(2):306-12
pubmed: 21450454
J Neurosci. 1996 Apr 1;16(7):2157-63
pubmed: 8601796
Neuron. 2007 Mar 1;53(5):719-34
pubmed: 17329211
Neuroreport. 1995 Dec 15;6(18):2553-6
pubmed: 8741761
Methods Mol Biol. 2011;681:259-80
pubmed: 20978970
Science. 1995 Sep 22;269(5231):1737-40
pubmed: 7569905
Mol Brain. 2014 Sep 03;7:64
pubmed: 25182223
Neuron. 2018 Mar 7;97(5):1094-1109.e9
pubmed: 29429936
Neuron. 1998 Jan;20(1):125-34
pubmed: 9459448
Front Mol Neurosci. 2008 Mar 28;1:4
pubmed: 18946537
Neuron. 2014 Jun 4;82(5):1101-14
pubmed: 24908489
PLoS One. 2011;6(8):e23069
pubmed: 21829694
Protein Sci. 2021 Nov;30(11):2246-2257
pubmed: 34538002