Do frozen embryo transfers modify the epigenetic control of imprinted genes and transposable elements in newborns compared with fresh embryo transfers and natural conceptions?
Adult
Cohort Studies
Cryopreservation
/ methods
DNA Methylation
/ genetics
DNA Transposable Elements
/ genetics
Embryo Transfer
/ methods
Epigenesis, Genetic
/ genetics
Female
Fertilization
/ genetics
Fertilization in Vitro
/ methods
Genomic Imprinting
/ genetics
Humans
Infant, Newborn
Placenta
/ physiology
Pregnancy
Prospective Studies
DNA methylation
frozen embryo transfer
imprinted genes
singletons
transposable elements
Journal
Fertility and sterility
ISSN: 1556-5653
Titre abrégé: Fertil Steril
Pays: United States
ID NLM: 0372772
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
received:
17
05
2021
revised:
02
07
2021
accepted:
06
08
2021
pubmed:
21
9
2021
medline:
22
12
2021
entrez:
20
9
2021
Statut:
ppublish
Résumé
To determine whether the epigenetic control of imprinted genes (IGs) and transposable elements (TEs) differs at birth between fresh or frozen embryo transfers and natural conceptions. Prospective study. University hospital. A total of 202 singleton births were divided into three groups: 84 natural pregnancies (controls), 66 in vitro fertilization/intracytoplasmic sperm injection with fresh embryo transfers, and 52 vitro fertilization/intracytoplasmic sperm injection with frozen embryo transfers. None. Pyrosequencing was used to assess the DNA methylation profiles of three IGs (H19/IGF2:IG-DMR [two sequences], KCNQ1OT1:TSS-DMR, and SNURF:TSS-DMR) and two TEs (LINE-1 and HERV-FRD) in cord blood and placenta. The quantitative reverse transcriptase polymerase chain reaction was used to study the transcription of three IGs (H19, KCNQ1, and SNRPN) and two TEs (LINE-1 and ORF2). After adjustment, the placental DNA methylation levels of H19/IGF2 were lower in the fresh embryo transfer group than in the control (H19/IGF2-seq1) and frozen embryo transfer (H19/IGF2-seq2) groups. The DNA methylation rate for LINE-1 was lower in placentas from the fresh embryo transfer group than in placentas from the control and frozen embryo transfer groups and for HERV-FRD compared with controls. In cord blood, DNA methylation levels were not significantly associated with the mode of conception. The relative expression of LINE-1 and ORF2 was decreased in both cord blood and placental tissues from fresh embryo transfer conceptions compared with natural conceptions and frozen embryo transfer conceptions. Compared with natural conceptions and frozen embryo transfers, fresh embryo transfers were associated with methylation and/or transcription changes in some TEs and IGs, mostly in placental samples, which could indicate altered placental epigenetic regulation resulting from ovarian stimulation protocols.
Identifiants
pubmed: 34538459
pii: S0015-0282(21)01937-3
doi: 10.1016/j.fertnstert.2021.08.014
pii:
doi:
Substances chimiques
DNA Transposable Elements
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1468-1480Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2021 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.