Isolation of Single Cells from Mouse Periodontal Ligament.
Cementoblasts
Mouse periodontal ligament
Single cells
Stem cells
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Aug 2021
20 Aug 2021
Historique:
received:
16
02
2021
revised:
24
04
2021
accepted:
26
04
2021
entrez:
20
9
2021
pubmed:
21
9
2021
medline:
21
9
2021
Statut:
epublish
Résumé
The periodontal ligament (PDL) is an essential tissue connecting teeth and bone. It is a complex tissue specifically designed to absorb the forces of mastication; analysis of its multiple cell populations is important to understand its function and the cell changes associated with periodontal disease. Cells in the periodontal ligament are not fully understood due to their physical location and small tissue size. It is challenging to isolate thin layers of cells compared with many other more substantial tissues. Here, we provide a straightforward protocol for the isolation of periodontal ligament cells from mice.
Identifiants
pubmed: 34541039
doi: 10.21769/BioProtoc.4120
pii: e4120
pmc: PMC8413550
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e4120Informations de copyright
Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThere are no conflicts of interest or competing interests.
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