Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila.

Animals Central Nervous System Drosophila Proteins / genetics Drosophila melanogaster Microfilament Proteins / genetics
Actin-binding protein Confocal microscopy Drosophila Dunc-115 MARCM

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 20 9 2021
pubmed: 21 9 2021
medline: 8 1 2022
Statut: ppublish

Résumé

Using Drosophila actin-binding protein Dunc-115 as an example, this chapter describes a MARCM (mosaic analysis with a repressible cell marker)-based method for analyzing cytoskeletal components for their functions in the nervous system. Following a concise description about the principle, a step-by-step protocol is provided for generating the needed stocks and for histological analysis. Additional details and explanations have been given in the accompanying notes. Together, this should form a practical and sufficient recipe for performing at the single cell level loss-of-function and gain-of-function analyses of proteins associated with the cytoskeleton.

Identifiants

DOI: 10.1007/978-1-0716-1661-1_17 PMID: 34542862
pubmed: 34542862
doi: 10.1007/978-1-0716-1661-1_17
doi:

Substances chimiques

Drosophila Proteins 0
Microfilament Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

341-347

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Huber AB, Kolodkin AL, Ginty DD, Cloutier JF (2003) Signaling at the growth cone: ligand-receptor complexes and the control of axon growth and guidance. Annu Rev Neurosci 26:509–563
doi: 10.1146/annurev.neuro.26.010302.081139
Stoeckli E (2018) Understanding axon guidance: are we nearly there yet? Development 145:1–10
doi: 10.1242/dev.151415
Muñoz-Lasso DC, Romá-Mateo C, Pallardó FV, Gonzalez-Cabo P (2020) Much more than a scaffold: cytoskeletal proteins in neurological disorders. Cell 9:358–399
doi: 10.3390/cells9020358
Garcia MC, Abbasi M, Singh S, He Q (2007) Role of Drosophila gene dunc-115 in nervous system. Invertebr Neurosci 7:119–128
doi: 10.1007/s10158-007-0047-1
Roblodowski C, He Q (2017) Drosophila Dunc-115 mediates axon projection through actin binding. Invertebr Neurosci 17:1–8
doi: 10.1007/s10158-017-0195-x
Lee T, Luo L (2001) Mosaic analysis with a repressible cell marker (MARCM) for Drosophila neural development. Trends Neurosci 24:251–254
doi: 10.1016/S0166-2236(00)01791-4
Wu JS, Luo L (2006) A protocol for mosaic analysis with a repressible cell marker (MARCM) in Drosophila. Nat Protoc 1:2583–2589
doi: 10.1038/nprot.2006.320
Kunes S, Wilson C, Steller H (1993) Independent guidance of retinal axons in the developing visual system of Drosophila J. Neuroscience 13:752–767
doi: 10.1523/JNEUROSCI.13-02-00752.1993
Xu T, Rubin GM (1993) Analysis of genetic mosaics in developing and adult Drosophila tissues. Development 117:1223–1237
doi: 10.1242/dev.117.4.1223

Auteurs

Qi He (Q)

Department of Biology, Brooklyn College of the City University of New York, Brooklyn, NY, USA. QHe@brooklyn.cuny.edu.

Christopher Roblodowski (C)

Department of Biological Sciences and Geology, Queensborough Community College of the City University of New York, Bayside, NY, USA.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Functional Analysis of Actin-Binding Proteins...
questionsmedicales.fr › Système nerveux › Système nerveux central › Functional Analysis of Actin-Binding Proteins...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Protéines d'insecte › Protéines de Drosophila › Functional Analysis of Actin-Binding Proteins...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Drosophila melanogaster › Functional Analysis of Actin-Binding Proteins...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du cytosquelette › Protéines des microfilaments › Functional Analysis of Actin-Binding Proteins...