Translational targeting of inflammation and fibrosis in frozen shoulder: Molecular dissection of the T cell/IL-17A axis.


Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
28 09 2021
Historique:
accepted: 21 07 2021
entrez: 21 9 2021
pubmed: 22 9 2021
medline: 5 10 2021
Statut: ppublish

Résumé

Frozen shoulder is a common fibroproliferative disease characterized by the insidious onset of pain and restricted range of shoulder movement with a significant socioeconomic impact. The pathophysiological mechanisms responsible for chronic inflammation and matrix remodeling in this prevalent fibrotic disorder remain unclear; however, increasing evidence implicates dysregulated immunobiology. IL-17A is a key cytokine associated with inflammation and tissue remodeling in numerous musculoskeletal diseases, and thus, we sought to determine the role of IL-17A in the immunopathogenesis of frozen shoulder. We demonstrate an immune cell landscape that switches from a predominantly macrophage population in nondiseased tissue to a T cell-rich environment in disease. Furthermore, we observed a subpopulation of IL-17A-producing T cells capable of inducing profibrotic and inflammatory responses in diseased fibroblasts through enhanced expression of the signaling receptor IL-17RA, rendering diseased cells more sensitive to IL-17A. We further established that the effects of IL-17A on diseased fibroblasts was TRAF-6/NF-κB dependent and could be inhibited by treatment with an IKKβ inhibitor or anti-IL-17A antibody. Accordingly, targeting of the IL-17A pathway may provide future therapeutic approaches to the management of this common, debilitating disease.

Identifiants

pubmed: 34544860
pii: 2102715118
doi: 10.1073/pnas.2102715118
pmc: PMC8488623
pii:
doi:

Substances chimiques

Cytokines 0
IL17A protein, human 0
Interleukin-17 0
NF-kappa B 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Medical Research Council
ID : MR/R020515/1
Pays : United Kingdom

Informations de copyright

Copyright © 2021 the Author(s). Published by PNAS.

Déclaration de conflit d'intérêts

Competing interest statement: I.B.M. has received personal fees from AbbVie, Bristol Myers Squibb, Celgene, Janssen, Lilly, Novartis, UCB, and LEO Pharma, grants from Bristol Myers Squibb, Janssen, UCB, AstraZeneca, and Boehringer Ingelheim, and is a shareholder of Causeway Therapeutics. N.L.M. has received personal fees from AbbVie, Novartis, and Stryker, and is a shareholder of Causeway Therapeutics. The other authors declare no competing interests.

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Auteurs

Moeed Akbar (M)

Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow G12 8QQ, United Kingdom.

Lindsay A N Crowe (LAN)

Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow G12 8QQ, United Kingdom.

Michael McLean (M)

Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow G12 8QQ, United Kingdom.
Department of Orthopaedic Surgery, Queen Elizabeth University Hospital, Glasgow G51 4TF, United Kingdom.

Emma Garcia-Melchor (E)

Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow G12 8QQ, United Kingdom.

Lucy MacDonald (L)

Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow G12 8QQ, United Kingdom.

Kristyn Carter (K)

Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow G12 8QQ, United Kingdom.

Umberto G Fazzi (UG)

Department of Orthopaedic Surgery, Queen Elizabeth University Hospital, Glasgow G51 4TF, United Kingdom.

David Martin (D)

Department of Orthopaedic Surgery, Queen Elizabeth University Hospital, Glasgow G51 4TF, United Kingdom.

Angus Arthur (A)

Department of Orthopaedic Surgery, Queen Elizabeth University Hospital, Glasgow G51 4TF, United Kingdom.

James H Reilly (JH)

Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow G12 8QQ, United Kingdom.

Iain B McInnes (IB)

Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow G12 8QQ, United Kingdom.

Neal L Millar (NL)

Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences,University of Glasgow, Glasgow G12 8QQ, United Kingdom; neal.millar@glasgow.ac.uk.
Department of Orthopaedic Surgery, Queen Elizabeth University Hospital, Glasgow G51 4TF, United Kingdom.

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