Neuroprotective effect of sumatriptan in pentylenetetrazole-induced seizure is mediated through N-methyl-D-aspartate/nitric oxide and cAMP response element-binding protein signaling pathway.
N-methyl-D-aspartate
cAMP response element-binding protein
mice
nitric oxide
pentylenetetrazole
seizure
sumatriptan
Journal
Fundamental & clinical pharmacology
ISSN: 1472-8206
Titre abrégé: Fundam Clin Pharmacol
Pays: England
ID NLM: 8710411
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
revised:
29
08
2021
received:
13
06
2021
accepted:
17
09
2021
pubmed:
22
9
2021
medline:
16
3
2022
entrez:
21
9
2021
Statut:
ppublish
Résumé
Seizure occurs as a result of uncontrolled electrical disturbances within the brain. Various biomolecules such as N-methyl-D-aspartate (NMDA), nitric oxide (NO), and cAMP response element-binding protein (CREB) have been implicated in the pathophysiology of seizure. Sumatriptan is a specific 5-Hydroxytryptamine 1B/1D receptor agonist and has neuroprotective effects in various neuropsychiatric disorders. In the current study, we tried to investigate the possible interaction of sumatriptan with NMDA/NO and CREB signaling pathway in PTZ induced seizure. For this purpose, various agonist and antagonist of NMDA such as MK-801 and Ketamine, NO precursor L-ARG, and NOS inhibitors L-NAME and 7-NI were co-administered with sumatriptan in PTZ induced seizure model. The level of nitrite in mice hippocampus was determined by Griess reaction. The gene expression of NR1, NR2A, NR2B, and CREB were quantified by quantitative real time-polymerase chain reaction (qRT-PCR). Furthermore, the involved neuronal nitric oxide synthase (nNOS) protein expression was examined via western blot analysis. Effective dose of sumatriptan (1.2 mg/kg) alone and subeffective dose of sumatriptan (0.3 mg/kg) in combination with NMDA and/or NO antagonist showed significant (P < 0.001) anticonvulsant activity in mice. Furthermore, sumatriptan significantly inhibited the PTZ-induced mRNA expression of NR2A (P < 0.0001), NR2B (P < 0.05), and CREB (P < 0.01). Also, the expression of nNOS protein in PTZ treated group was reversed by sumatriptan (P < 0.01). Hence, current findings suggest that the anticonvulsant effect of sumatriptan was due to down regulation of NMDA/NO and CREB signaling pathway.
Substances chimiques
Cyclic AMP Response Element-Binding Protein
0
Neuroprotective Agents
0
Nitric Oxide
31C4KY9ESH
N-Methylaspartate
6384-92-5
Sumatriptan
8R78F6L9VO
Pentylenetetrazole
WM5Z385K7T
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
250-261Subventions
Organisme : Iran National Science Foundation (INSF)
ID : (Grant No. 96002757)
Organisme : International Campus of Tehran University of Medical Sciences, Tehran, Iran
ID : (Grant number
Organisme : International Campus of Tehran University of Medical Sciences, Tehran, Iran
ID : 97-01-103-38095)
Informations de copyright
© 2021 Société Française de Pharmacologie et de Thérapeutique.
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