In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells.
human pluripotent stem cells (hPSCs)
motor neurons
neuromesodermal progenitors
Journal
Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
entrez:
21
9
2021
pubmed:
22
9
2021
medline:
24
9
2021
Statut:
ppublish
Résumé
The ability to generate spinal cord motor neurons from human pluripotent stem cells (hPSCs) is of great use for modelling motor neuron-based diseases and cell-replacement therapies. A key step in the design of hPSC differentiation strategies aiming to produce motor neurons involves induction of the appropriate anteroposterior (A-P) axial identity, an important factor influencing motor neuron subtype specification, functionality, and disease vulnerability. Most current protocols for induction of motor neurons from hPSCs produce predominantly cells of a mixed hindbrain/cervical axial identity marked by expression of Hox paralogous group (PG) members 1-5, but are inefficient in generating high numbers of more posterior thoracic/lumbosacral Hox PG(8-13)
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e244Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P000444/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/V002163/1
Pays : United Kingdom
Informations de copyright
© 2021 The Authors. Current Protocols published by Wiley Periodicals LLC.
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