Synthesis and characterization of tracers and development of a fluorescence polarization immunoassay for amantadine with high sensitivity in chicken.
amantadine
chicken
fluorescence polarization immunoassay
sensitivity
tracer structure
Journal
Journal of food science
ISSN: 1750-3841
Titre abrégé: J Food Sci
Pays: United States
ID NLM: 0014052
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
revised:
24
07
2021
received:
24
04
2021
accepted:
02
08
2021
pubmed:
23
9
2021
medline:
21
10
2021
entrez:
22
9
2021
Statut:
ppublish
Résumé
Fluorescence polarization immunoassay (FPIA) is a homogeneous and rapid analytical method that is suitable for high-throughput screening of large numbers of samples. However, FPIA typically suffers from lower sensitivity than the well-established enzyme-linked immunosorbent assay (ELISA), limiting its wide application as an analytical tool that can be run with trace levels of an analyte. Herein, a highly sensitive FPIA for detecting amantadine (AMD) in chicken is described. To achieve high sensitivity, nine chemical tracers of AMD that employ different fluoresceins, fluorescein derivatives, and haptens were synthesized and paired with four previously produced monoclonal antibodies (mAbs). The effect of the tracer structure on the sensitivity of FPIA was investigated and discussed. We found that the tracers with a linear and shorter bridge between adamantane and fluorescein generally provided higher sensitivity. After optimization, N'-(1-adamantyl) ethylenediamine (AEDA), an AMD structural analogue labeled with fluorescein isothiocyanate (FITC), achieved the lowest IC
Identifiants
pubmed: 34549423
doi: 10.1111/1750-3841.15896
doi:
Substances chimiques
Amantadine
BF4C9Z1J53
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4754-4767Informations de copyright
© 2021 Institute of Food Technologists®.
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