Assembly of long L-RNA by native RNA ligation.
Journal
Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838
Informations de publication
Date de publication:
12 Oct 2021
12 Oct 2021
Historique:
pubmed:
23
9
2021
medline:
30
11
2021
entrez:
22
9
2021
Statut:
epublish
Résumé
Due to their intrinsic nuclease resistance, L-oligonucleotides are being increasingly utilized in the development of molecular tools and sensors. Yet, it remains challenging to synthesize long L-oligonucleotides, potential limiting future applications. Herein, we report straightforward and versitile approach to assemble long L-RNAs from two or more shorter fragments using T4 RNA ligase 1. We show that this approach is compatible with the assembly of several classes of functional L-RNA, which we highlight by generating a 124 nt L-RNA biosensor that functions in serum.
Identifiants
pubmed: 34550128
doi: 10.1039/d1cc04296c
pmc: PMC8641125
mid: NIHMS1756061
doi:
Substances chimiques
Viral Proteins
0
RNA
63231-63-0
RNA Ligase (ATP)
EC 6.5.1.3
bacteriophage T4 RNA ligase 1
EC 6.5.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
10508-10511Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM124974
Pays : United States
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