Cellular bases of olfactory circuit assembly revealed by systematic time-lapse imaging.
time-lapse imaging, circuit assembly, olfactory system, olfactory receptor neurons, antennal lobe, Drosophilia, growth cone, cytoskeleton, bilateral symmetry, target selection
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
30 09 2021
30 09 2021
Historique:
received:
23
04
2021
revised:
21
07
2021
accepted:
24
08
2021
pubmed:
23
9
2021
medline:
8
1
2022
entrez:
22
9
2021
Statut:
ppublish
Résumé
Neural circuit assembly features simultaneous targeting of numerous neuronal processes from constituent neuron types, yet the dynamics is poorly understood. Here, we use the Drosophila olfactory circuit to investigate dynamic cellular processes by which olfactory receptor neurons (ORNs) target axons precisely to specific glomeruli in the ipsi- and contralateral antennal lobes. Time-lapse imaging of individual axons from 30 ORN types revealed a rich diversity in extension speed, innervation timing, and ipsilateral branch locations and identified that ipsilateral targeting occurs via stabilization of transient interstitial branches. Fast imaging using adaptive optics-corrected lattice light-sheet microscopy showed that upon approaching target, many ORN types exhibiting "exploring branches" consisted of parallel microtubule-based terminal branches emanating from an F-actin-rich hub. Antennal nerve ablations uncovered essential roles for bilateral axons in contralateral target selection and for ORN axons to facilitate dendritic refinement of postsynaptic partner neurons. Altogether, these observations provide cellular bases for wiring specificity establishment.
Identifiants
pubmed: 34551316
pii: S0092-8674(21)01013-8
doi: 10.1016/j.cell.2021.08.030
pmc: PMC8545656
mid: NIHMS1736620
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
5107-5121.e14Subventions
Organisme : NIDCD NIH HHS
ID : K99 DC018830
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC005982
Pays : United States
Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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