A High-Throughput Method to Analyze the Interaction Proteins With p22 Protein of African Swine Fever Virus
African swine fever virus
GO KEGG pathways analysis
liquid chromatography
mass spectrometry
protein p22
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2021
2021
Historique:
received:
03
06
2021
accepted:
03
08
2021
entrez:
23
9
2021
pubmed:
24
9
2021
medline:
24
9
2021
Statut:
epublish
Résumé
African swine fever virus (ASFV) has been identified as the agent of African swine fever, resulting in a mortality rate of nearly 100% in domestic pigs worldwide. Protein p22 encoded by gene KP177R has been reported to be localized at the inner envelope of the virus, while the function of p22 remains unclear. In this study, p22 interacting proteins of the host were identified by a high-throughput method and analyzed by Gene ontology terms and Kyoto Encyclopedia of Gene and Genomes (KEGG) pathways; numerous cellular proteins in 293-T that interacted with p22 protein were identified. These interacting proteins were related to the biological processes of binding, cell structure, signal transduction, cell adhesion, etc. At the same time, the interacted proteins participated in several KEGG pathways like ribosome, spliceosome, etc. The key proteins in the protein-protein interaction network were closely related to actin filament organization and movement, resulting in affecting the process of phagocytosis and endocytosis. A large number of proteins that interacted with p22 were identified, providing a large database, which should be very useful to elucidate the function of p22 in the near future, laying the foundation for elucidating the mechanism of ASFV.
Identifiants
pubmed: 34552974
doi: 10.3389/fvets.2021.719859
pmc: PMC8450437
doi:
Types de publication
Journal Article
Langues
eng
Pagination
719859Informations de copyright
Copyright © 2021 Zhu, Fan, Zhou, Wang, Fan and Li.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
PLoS Pathog. 2016 Apr 25;12(4):e1005595
pubmed: 27110717
J Virol. 2009 Dec;83(23):12290-300
pubmed: 19793823
J Virol. 2018 Nov 12;92(23):
pubmed: 30185597
Vet Microbiol. 2014 Jan 31;168(2-4):413-9
pubmed: 24398227
Gene. 2021 Feb 5;768:145307
pubmed: 33197516
J Virol. 2016 Dec 16;91(1):
pubmed: 27795439
Virus Res. 2013 Apr;173(1):122-30
pubmed: 23137735
Small GTPases. 2018 Jul 4;9(4):349-351
pubmed: 27687479
J Cell Sci. 2005 Mar 1;118(Pt 5):843-6
pubmed: 15731001
Virology. 1991 Mar;181(1):251-7
pubmed: 1994575
Fish Shellfish Immunol. 2018 Aug;79:65-72
pubmed: 29753142
Viruses. 2021 May 26;13(6):
pubmed: 34073222
Gene. 2018 Jul 20;664:152-167
pubmed: 29679756
J Virol. 2011 Apr;85(7):3294-305
pubmed: 21248037
Virus Res. 2013 Apr;173(1):191-7
pubmed: 23123296
Nat Cell Biol. 2000 Apr;2(4):246-8
pubmed: 10783245
Philos Trans R Soc Lond B Biol Sci. 2019 May 27;374(1773):20180292
pubmed: 30955495
J Virol. 2010 Aug;84(15):7484-99
pubmed: 20504920
Nat Rev Mol Cell Biol. 2013 Jan;14(1):7-12
pubmed: 23212475
Emerg Microbes Infect. 2019;8(1):438-447
pubmed: 30898043
Cells. 2019 Nov 13;8(11):
pubmed: 31766144
Virology. 1995 Apr 1;208(1):249-78
pubmed: 11831707
Nucleic Acids Res. 2015 Jan;43(Database issue):D1049-56
pubmed: 25428369
Dev Cell. 2017 Sep 11;42(5):498-513.e6
pubmed: 28867487
Mol Cell. 2004 Oct 22;16(2):269-79
pubmed: 15494313
Virus Res. 2013 Apr;173(1):180-90
pubmed: 23313935
Mol Pharmacol. 2018 Apr;93(4):270-276
pubmed: 29217670
J Gen Virol. 1998 May;79 ( Pt 5):1179-88
pubmed: 9603333
Lipids Health Dis. 2019 Nov 4;18(1):191
pubmed: 31684957
Mol Nutr Food Res. 2021 Jan;65(2):e2000810
pubmed: 33200558
Emerg Infect Dis. 2011 Apr;17(4):599-605
pubmed: 21470447
Virus Res. 2013 Dec 26;178(2):328-39
pubmed: 24076499
Virus Res. 2013 Apr;173(1):3-14
pubmed: 23142553
Nucleic Acids Res. 2000 Jan 1;28(1):27-30
pubmed: 10592173
Virus Res. 2013 Apr;173(1):29-41
pubmed: 23059353
Int J Mol Sci. 2019 May 05;20(9):
pubmed: 31060328
Gastroenterol Hepatol Bed Bench. 2014 Winter;7(1):17-31
pubmed: 25436094