Integrin α5β1 nano-presentation regulates collective keratinocyte migration independent of substrate rigidity.
Cell Adhesion
Cell Culture Techniques
Cell Movement
Cell Proliferation
Cell-Matrix Junctions
/ metabolism
Extracellular Matrix
/ metabolism
Fibronectins
/ metabolism
HaCaT Cells
Humans
Hydrogels
Integrin alpha5beta1
/ metabolism
Keratinocytes
/ metabolism
Mechanotransduction, Cellular
Nanostructures
Surface Properties
Time Factors
Wound Healing
cell biology
collective cell migration
human
integrin α5β1
keratocytes
physics of living systems
wound healing
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
23 09 2021
23 09 2021
Historique:
received:
28
04
2021
accepted:
13
09
2021
entrez:
23
9
2021
pubmed:
24
9
2021
medline:
21
10
2021
Statut:
epublish
Résumé
Nanometer-scale properties of the extracellular matrix influence many biological processes, including cell motility. While much information is available for single-cell migration, to date, no knowledge exists on how the nanoscale presentation of extracellular matrix receptors influences collective cell migration. In wound healing, basal keratinocytes collectively migrate on a fibronectin-rich provisional basement membrane to re-epithelialize the injured skin. Among other receptors, the fibronectin receptor integrin α5β1 plays a pivotal role in this process. Using a highly specific integrin α5β1 peptidomimetic combined with nanopatterned hydrogels, we show that keratinocyte sheets regulate their migration ability at an optimal integrin α5β1 nanospacing. This efficiency relies on the effective propagation of stresses within the cell monolayer independent of substrate stiffness. For the first time, this work highlights the importance of extracellular matrix receptor nanoscale organization required for efficient tissue regeneration.
Identifiants
pubmed: 34554089
doi: 10.7554/eLife.69861
pii: 69861
pmc: PMC8460267
doi:
pii:
Substances chimiques
Fibronectins
0
Hydrogels
0
Integrin alpha5beta1
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021, Di Russo et al.
Déclaration de conflit d'intérêts
JD, JW, TS, HK, JS none, JY None
Références
Nat Cell Biol. 2013 Jun;15(6):625-36
pubmed: 23708002
J Cell Sci. 2008 Jan 15;121(Pt 2):196-204
pubmed: 18187451
J Invest Dermatol. 1993 Oct;101(4):600-4
pubmed: 8409530
Physiology (Bethesda). 2013 Nov;28(6):370-9
pubmed: 24186932
Angew Chem Int Ed Engl. 2016 Jun 13;55(25):7048-67
pubmed: 27258759
J Cell Biol. 1989 Aug;109(2):903-14
pubmed: 2760116
Adv Wound Care (New Rochelle). 2014 Dec 1;3(12):762-783
pubmed: 25493210
Angew Chem Int Ed Engl. 2013 Oct 25;52(44):11656-9
pubmed: 24115324
Science. 2016 Sep 9;353(6304):1157-61
pubmed: 27609894
Biophys J. 2007 Apr 15;92(8):2964-74
pubmed: 17277192
Nature. 2017 Dec 14;552(7684):219-224
pubmed: 29211717
Eur J Cell Biol. 2006 Apr;85(3-4):219-24
pubmed: 16546564
J Cell Biol. 2000 Sep 4;150(5):1149-60
pubmed: 10974002
J Invest Dermatol. 2018 Mar;138(3):627-636
pubmed: 29080682
Nat Cell Biol. 2015 Mar;17(3):276-87
pubmed: 25706233
Angew Chem Int Ed Engl. 2013 Jan 28;52(5):1572-5
pubmed: 23345131
Colloids Surf B Biointerfaces. 2015 Apr 1;128:191-200
pubmed: 25637448
Nat Commun. 2018 Aug 27;9(1):3469
pubmed: 30150695
Cell Mol Life Sci. 2012 Aug;69(16):2779-89
pubmed: 22410739
Nat Rev Mol Cell Biol. 2017 Dec;18(12):743-757
pubmed: 29115298
J Biol Chem. 2001 Jun 1;276(22):18878-87
pubmed: 11278780
J Vis Exp. 2010 Dec 14;(46):
pubmed: 21178972
Nature. 2018 Feb 22;554(7693):523-527
pubmed: 29443958
J Cell Biol. 2013 Nov 25;203(4):691-709
pubmed: 24385489
EMBO Rep. 2012 Nov 6;13(11):984-91
pubmed: 23059978
Development. 1993 Dec;119(4):1093-105
pubmed: 7508365
Sci Rep. 2017 Jan 11;7:39805
pubmed: 28074920
J Cell Biol. 2012 Oct 29;199(3):545-63
pubmed: 23091067
Adv Mater. 2015 Jul 1;27(25):3737-47
pubmed: 25981929
HFSP J. 2008 Oct;2(5):276-85
pubmed: 19404439
Trends Cell Biol. 2011 Nov;21(11):638-46
pubmed: 21784638
Proc Natl Acad Sci U S A. 2012 May 1;109(18):6933-8
pubmed: 22509005
Mol Biol Cell. 2017 Jul 7;28(14):1901-1911
pubmed: 28468976
J Cell Biol. 2006 Jan 16;172(2):259-68
pubmed: 16401722
Nat Cell Biol. 2015 Apr;17(4):409-20
pubmed: 25812522
Soft Matter. 2020 Feb 21;16(7):1825-1839
pubmed: 31970382
Science. 2009 Jan 30;323(5914):638-41
pubmed: 19179532
Chemphyschem. 2004 Mar 19;5(3):383-8
pubmed: 15067875
Life Sci Alliance. 2020 Nov 30;4(1):
pubmed: 33257474
J Cell Biol. 1993 Jul;122(1):235-42
pubmed: 8314844
Nat Mater. 2011 Jun;10(6):469-75
pubmed: 21602808
Development. 2002 May;129(9):2303-15
pubmed: 11959837