A simple pooling salivary test for SARS-CoV-2 diagnosis: A Columbus' egg?
SARS-CoV-2
qPCR
saliva
Journal
Virus research
ISSN: 1872-7492
Titre abrégé: Virus Res
Pays: Netherlands
ID NLM: 8410979
Informations de publication
Date de publication:
11 2021
11 2021
Historique:
received:
17
06
2021
revised:
30
08
2021
accepted:
13
09
2021
pubmed:
25
9
2021
medline:
17
12
2022
entrez:
24
9
2021
Statut:
ppublish
Résumé
Saliva is an appropriate specimen for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) diagnosis. The possibility of pooling samples of saliva, using non-invasive bibula strips for sampling, was explored employing Bovine coronavirus (BCoV) spiked saliva. In laboratory, up to 30 saliva-soaked strips were pooled in a single tube with 2 mL of medium. After quick adsorption with the medium and vortexing, the liquid was collected and tested with a quantitative molecular assay to quantify viral RNA genome copies. On testing of single and pooled strips, the difference between the median threshold cycles (Ct) value of test performed on the single positive saliva sample and the median Ct value obtained on the pool of 30 strips, was 3.21 cycles. Saliva pooling with bibula strips could allow monitoring of COVID-19 on a large scale, reducing costs for the health bodies in terms of medical material and skilled personnel. Finally, saliva sampling is noninvasive and less traumatic than nasopharyngeal swabs and can be self-collected.
Identifiants
pubmed: 34560181
pii: S0168-1702(21)00282-3
doi: 10.1016/j.virusres.2021.198575
pmc: PMC8454190
pii:
doi:
Substances chimiques
RNA, Viral
0
Reagent Strips
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
198575Informations de copyright
Copyright © 2021. Published by Elsevier B.V.
Références
Clin Infect Dis. 2020 Dec 31;71(11):2939-2946
pubmed: 32562544
Int J Infect Dis. 2021 Apr;105:83-90
pubmed: 33581365
N Engl J Med. 2020 Sep 24;383(13):1283-1286
pubmed: 32857487
Front Med (Lausanne). 2020 Aug 04;7:465
pubmed: 32903849
J Epidemiol Glob Health. 2021 Mar;11(1):55-59
pubmed: 32959618
J Med Virol. 2021 Mar;93(3):1506-1511
pubmed: 32841429
Clin Infect Dis. 2022 Mar 1;74(4):719-722
pubmed: 33993225
J Oral Sci. 2020;62(3):350-351
pubmed: 32581183
Clin Infect Dis. 2020 Jul 28;71(15):841-843
pubmed: 32047895
J Clin Microbiol. 2020 Aug 24;58(9):
pubmed: 32636214
J Virol Methods. 2005 Dec;130(1-2):72-8
pubmed: 16024100
J Clin Microbiol. 2020 Jul 23;58(8):
pubmed: 32393482
J Virol Methods. 2008 Aug;151(2):167-171
pubmed: 18579223
J Chin Med Assoc. 2020 Oct;83(10):891-894
pubmed: 32773584
Lancet Infect Dis. 2020 Nov;20(11):1231-1232
pubmed: 32530425
Biometrics. 2012 Mar;68(1):287-96
pubmed: 21762119
Biosens Bioelectron. 2020 Oct 15;166:112455
pubmed: 32739797
Emerg Infect Dis. 2021 Apr;27(4):
pubmed: 33755009
J Infect. 2020 Jul;81(1):e45-e50
pubmed: 32298676
Int J Mol Med. 2021 Jun;47(6):
pubmed: 33846767
Lancet Infect Dis. 2020 May;20(5):565-574
pubmed: 32213337