Bioactive chitosan biguanidine-based injectable hydrogels as a novel BMP-2 and VEGF carrier for osteogenesis of dental pulp stem cells.
Bone Morphogenetic Protein 2
/ chemistry
Cell Differentiation
/ drug effects
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Cells, Cultured
Chitosan
/ analogs & derivatives
Dental Pulp
/ cytology
Drug Carriers
/ chemistry
Drug Liberation
Guanidines
/ chemistry
Humans
Hydrogels
/ chemistry
Osteoblasts
/ metabolism
Osteogenesis
/ drug effects
Recombinant Proteins
/ chemistry
Stem Cells
/ drug effects
Tensile Strength
Tissue Scaffolds
/ chemistry
Transforming Growth Factor beta
/ chemistry
Vascular Endothelial Growth Factor A
/ chemistry
Bone scaffold
Bone tissue engineering
Chitosan hydrogel
Growth factor
Journal
Carbohydrate polymers
ISSN: 1879-1344
Titre abrégé: Carbohydr Polym
Pays: England
ID NLM: 8307156
Informations de publication
Date de publication:
01 Dec 2021
01 Dec 2021
Historique:
received:
22
02
2021
revised:
24
07
2021
accepted:
17
08
2021
entrez:
25
9
2021
pubmed:
26
9
2021
medline:
27
1
2022
Statut:
ppublish
Résumé
Nowadays, vascularization and mineralization of bone defects is the main bottleneck in the bone regeneration field that is needed to be overcome and developed. Here, we prepared novel in-situ formed injectable hydrogels based on chitosan biguanidine and carboxymethylcellulose loaded with vascular endothelial growth factor (VEGF) and recombinant Bone morphogenetic protein 2 (BMP-2) and studied its influence on osteoblastic differentiation of dental pulp stem cells (DPSCs). The sequential release behavior of the VEGF and BMP-2 from hydrogels adjusted with the pattern of normal human bone growth. MTT assay exhibited that these hydrogels were non-toxic and significantly increased DPSCs proliferation. The Real-time PCR and Western blot analysis on CG11/BMP2-VEGF showed significantly higher gene and protein expression of ALP, COL1α1, and OCN. These results were confirmed by mineralization assay by Alizarin Red staining and Alkaline phosphatase enzyme activity. Based on these evaluations, these hydrogel holds potential as an injectable bone tissue engineering platform.
Identifiants
pubmed: 34560990
pii: S0144-8617(21)00976-0
doi: 10.1016/j.carbpol.2021.118589
pii:
doi:
Substances chimiques
Bone Morphogenetic Protein 2
0
Drug Carriers
0
Guanidines
0
Hydrogels
0
Recombinant Proteins
0
Transforming Growth Factor beta
0
Vascular Endothelial Growth Factor A
0
recombinant human bone morphogenetic protein-2
0
Chitosan
9012-76-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
118589Informations de copyright
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